The proposed approach for determining catalytic activity of the SARS-CoV-2 main protease (M) is based on registration of the peak area of electrochemical oxidation of tyrosine residue in the model peptide substrate CGGGAVLQSGY immobilized on the surface of a graphite screen-printed electrode (SPE) modified with gold nanoparticles (AuNP). The AuNP were obtained by electrosynthesis. Steady state kinetic parameters of M in the reaction with the model peptide were determined: catalytic constant () was (3.1 ± 0.1) × 10 s; Michaelis constant () was (358 ± 32) × 10 M; catalytic efficiency (/) was 8659 s/M. The limit of detection (LOD) determined for M using the proposed electrochemical system was 44 nM. The proposed approach is a promising tool to search for new M inhibitors as drugs for treatment of coronavirus infections.
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