Recent advances in functional genomics have allowed identification of thousands of translated short open reading frames (sORFs) in the 5' leaders of mammalian mRNA transcripts. While most sORFs are unlikely to encode functional proteins, a small number have been shown to have evolved as protein-coding genes. As a result, dozens of these sORFs have already been annotated as protein-coding ORFs. mRNAs that contain both a protein-coding sORF and an annotated coding sequence (CDS) are referred to as bicistronic transcripts. In this study, we focus on three genes - , , and - which give rise to bicistronic mRNAs. We discuss recent findings regarding functional investigation of the corresponding polypeptide products, as well as how their translation is regulated, and how this unusual genetic arrangement may have evolved.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1134/S0006297924603630 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Unveiling the Pathogenic Role of Novel CPLANE1 Compound Heterozygous Variants in Joubert Syndrome: Insights Into mRNA Stability and NMD Pathway.
J Cell Mol Med
March 2025
Center for Reproductive Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, People's Republic of China.
Joubert syndrome (JS) is a rare neurodevelopmental disorder associated with mutations in genes involved in ciliary function. Germline variants in CPLANE1 have been implicated in JS. In this study, we investigated a family with three adverse pregnancies characterised by fetal malformations consistent with JS.
View Article and Find Full Text PDFZNF667 alleviates the inflammatory damage in intervertebral disc degeneration via inhibiting NF-κB signaling pathway.
Zhong Nan Da Xue Xue Bao Yi Xue Ban
October 2024
Department of Spine Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Objectives: With the aging population, the incidence of intervertebral disc degeneration (IDD) is increasing every year. The pathogenesis of IDD is complex, and there are currently no effective treatment options. This study aims to investigate the specific function and underlying mechanism of zinc finger protein 667 (ZNF667) in the inflammatory damage of nucleus pulposus cells in IDD.
View Article and Find Full Text PDFIn vitro study of a siRNA delivery liposome constructed with an ionizable cationic lipid.
Zhong Nan Da Xue Xue Bao Yi Xue Ban
October 2024
Department of Pharmaceutical Engineering, Chemistry and Chemical Engineering, Central South University, Changsha 410083.
Objectives: Small interfering RNA (siRNA) can silence disease-related genes through sequence-specific RNA interference (RNAi). Cationic lipid-based liposomes effectively deliver nucleic acids into the cytoplasm but often exhibit significant toxicity. This study aims to synthesize a novel ionizable lipid, Nε-laruoyl-lysine amide (LKA), from natural amino acids, constructed LKA-based liposomes, and perform physicochemical characterization and cell-based experiments to systematically evaluate the potential of these ionizable lipid-based liposomes for nucleic acid delivery.
View Article and Find Full Text PDFVoltage-gated ion channels in cultured gill epithelia of rainbow trout, Oncorhynchus mykiss, change in transcript abundance with exposure to freshwater.
Comp Biochem Physiol A Mol Integr Physiol
March 2025
Department of Biological Sciences, California State University San Marcos, CA 92096, USA. Electronic address:
Salmonid fishes are well adapted to transition between salinities as part of a diadromid lifestyle, and many species are both economically and environmentally important. Ion-transporting gill epithelium helps fishes maintain ion balance during salinity transition. Recent transcriptomic surveys suggest that voltage-gated ion channels (VGICs) are present in gill epithelium of fishes.
View Article and Find Full Text PDFmicroRNA-34: A multifunctional miRNA family.
Arch Biochem Biophys
March 2025
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, PR China. Electronic address:
MicroRNAs are endogenous non-coding small RNAs composed of about 22 nucleotides, which are widely found in eukaryotic cells and regulate gene expression at the post-transcriptional level through complementary pairing with target genes, leading to mRNA degradation or translation inhibition. miR-34 is a highly conserved miRNA during evolution. Recent studies have found that members of the miR-34 family are involved in regulating biological processes such as aging, ciliogenesis, and immunity.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!