Mitochondrion apart from being the energy hub of the cell, is also the center of various signaling pathways. During intracellular infection, either bacterial or viral, several pathogen proteins, metabolites, and possibly, lipids interact with the host mitochondria. These interactions allow the pathogens, such as Mtb, to reprogram the host mitochondrial functions, screwing the host immune responses and resulting in the persistence of the bacteria. Therefore, mitochondria offer a critical target organelle for various therapeutic interventions. This chapter deals with methods to demonstrate and establish the mitochondrial localization of Mtb proteins by confocal microscopy and mitochondrial enrichment. Transient transfection of mammalian constructs or infection Mycolicibacterium smegmatis infection could be used to overexpress the candidate Mtb protein in host cells, allowing the study of changes in the mitochondria's composition and function with regard to localization studies, mitochondrial DNA and RNA, proteomics, metabolomics, lipidomics, and bioenergetics.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/bs.mcb.2024.10.018 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Significant Tumor Inhibition of Trimethyl-152-[L-aspartyl]pheophorbide a in Tumor Photodynamic Therapy‡.
ChemMedChem
March 2025
Donghua University, Pharmaceutical Science & Technology, CHINA.
A novel pheophorbide derivative, trimethyl-152-[L-aspartyl]pheophorbide a was synthesised and investigated for anti-tumor activity. The prepared photosensitizer had good absorption in the phototherapeutic window and high ROS yields. It exhibited excellent phototoxicity higher than reference compound m-THPC when irradiated by 650 nm light in vitro, and obvious photodynamic anti-tumor effect in vivo.
View Article and Find Full Text PDFAggregatin is a mitochondrial regulator of MAVS activation to drive innate immunity.
J Immunol
February 2025
Department of Pharmacology and Toxicology, University of Arizona, Tucson, AZ, United States.
Mitochondrial antiviral-signaling protein (MAVS) is a key adapter protein required for inducing type I interferons (IFN-Is) and other antiviral effector molecules. The formation of MAVS aggregates on mitochondria is essential for its activation; however, the regulatory mitochondrial factor that mediates the aggregation process is unknown. Our recent work has identified the protein Aggregatin as a critical seeding factor for β-amyloid peptide aggregation.
View Article and Find Full Text PDFInvestigating cuproptosis and mitochondrial dysfunction in brain cells: uncovering novel mechanisms and biomarkers for Parkinson's disease.
Metab Brain Dis
March 2025
Xinxiang Key Laboratory of Targeted Intervention for Brain Cell Injury, Sanquan College of Xinxiang Medical University, Xinxiang, 453000, China.
Parkinson's disease (PD) is a chronic neurodegenerative condition marked by the gradual degeneration of dopaminergic neurons, resulting in a range of disabling motor and non-motor symptoms. Despite advances, the molecular mechanisms underlying PD remain elusive, and effective biomarkers and therapeutic targets are limited. Recent studies suggest that mitochondrial dysfunction and dysregulated cellular metabolism are central to PD pathogenesis.
View Article and Find Full Text PDFUCNPs@PVP-Hemin-GOx@CaCO Nanoplatform for Ferroptosis Self-Amplification Combined with Calcium Overload.
Adv Healthc Mater
March 2025
Molecular Diagnostic Center, Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang Province, Hangzhou First People's Hospital, Hangzhou, 310006, China.
Due to the complexity of the tumor microenvironment (TME), current tumor treatments cannot achieve satisfactory results. A nanocomposite material, UCNPs@PVP-Hemin-GOx@CaCO (UPHGC NPs) is developed that responds to the TME and controls release to achieve multimodal synergistic therapy in tumor tissues. UPHGC NPs mediate photodynamic therapy (PDT), chemodynamic therapy (CDT), and starvation therapy (ST) synergistically, ultimately inducing self-amplification of ferroptosis.
View Article and Find Full Text PDFInduction of mitochondrial damage via the CREB3L1/miR-34c/COX1 axis by porcine epidemic diarrhea virus infection facilitates pathogenicity.
J Virol
March 2025
College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Porcine epidemic diarrhea virus (PEDV) is a primary cause of viral diarrhea in neonatal piglets, leading to substantial economic losses in the swine industry globally. It primarily targets epithelial cells of the small intestine, compromising intestinal function and resulting in the death of affected animals. As mitochondria are essential for maintaining gut health, this study investigates the effects of PEDV infection on mitochondrial function in small intestinal epithelial cells and its subsequent impacts.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!