Green biotechnological synthesis of rare sugars/alcohols: D-allulose, allitol, D-tagatose, L-xylulose, L-ribose.

Rare sugars are paid more attention because of which have the characteristics of low calorie, low absorption and excellent physiological functions. Biotechnological synthesis of rare sugars has the advantages of being green, clean, simple and economic compared to chemical synthesis. Abundant enzymes for rare sugars biosynthesis are introduced and multienzyme cascade catalytic system (MECCS) used in biosynthesis of rare sugars is highlighted in this paper. Different biosynthesis pathways of five important rare sugars (D-allulose, allitol, D-tagatose, L-xylulose, l-ribose), mainly containing isomerization/epimerization reaction (existing thermodynamic equilibrium limitation), reduction-oxidation reaction (needing expensive cofactors) and phosphorylation-dephosphorylation reaction pathways (inherent constraint of thermodynamic equilibrium and requirement high-cost cofactors) etc., are reviewed. Furthermore, techniques of cofactor regeneration and enzyme/cell immobilization are provided. Finally, unique insights and expectations for future development in biosynthesis of rare sugars are given. This review provides a comprehensive analysis of the latest biotechnological advancements in the biosynthesis of rare sugars/alcohols, highlighting innovative multienzyme cascade catalytic systems and cofactor regeneration strategies.