Purpose: The purpose of this study was to investigate the influence of substrates under the three-dimensional periodontal ligament (3D PDL) tissue on its biological functions after compressive stimulation.
Methods: A 3D PDL tissue was created using a poly(L-lactic acid) (PLLA) porous scaffold impregnated with human periodontal ligament fibroblasts (hPDLFs). It was then placed on a polyetheretherketone (PEEK) substrate, which has a comparable elastic modulus to bone and was compressed (25 g/cm) for 1, 3, and 7 days. The morphology and biological functions of the hPDLFs in the 3D PDL tissue on the PEEK substrate were evaluated and compared with those on the polystyrene (PS) substrate.
Results: Compressive forces for the PLLA porous scaffold on the PEEK substrate were higher than those on the PS substrate. hPDLFs were present throughout the PLLA porous scaffold and there was no morphological change upon compressive stimulation. Increased expression of osteoclastogenic genes was observed after 3 days of compressive stimulation, while the level of these genes was increased by changing the substrate under the PDL tissue from PS to PEEK.
Conclusion: The substrate placed under the PDL tissue during compressive stimulation affects the biological functions of hPDLFs.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2334/josnusd.24-0366 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effect of substrate on the response of 3-dimensional periodontal ligament tissue to compressive stimulation.
J Oral Sci
March 2025
Department of Orthodontics, Osaka Dental University.
Purpose: The purpose of this study was to investigate the influence of substrates under the three-dimensional periodontal ligament (3D PDL) tissue on its biological functions after compressive stimulation.
Methods: A 3D PDL tissue was created using a poly(L-lactic acid) (PLLA) porous scaffold impregnated with human periodontal ligament fibroblasts (hPDLFs). It was then placed on a polyetheretherketone (PEEK) substrate, which has a comparable elastic modulus to bone and was compressed (25 g/cm) for 1, 3, and 7 days.
Evaluating the combination and comparison of ablative fractional lasers (CO2, Erbium-YAG) with pulsed dye laser (PDL) for treating hypertrophic scars: a systematic review.
Lasers Med Sci
March 2025
Department of Dermatology, Rasool Akram Medical Complex Clinical Research Development Center (RCRDC), School of Medicine, Iran University of Medical Sciences, Tehran, Iran, Tehran, Iran, Islamic Republic of.
The aim of this study is to compare pulsed dye laser (PDL) and ablative fractional lasers (CO2, Erbium-YAG) in the treatment of hypertrophic scars in a systematic review. Databases including Web of Science, Science Direct, Google Scholar and PubMed were searched for clinical trials up to December 1, 2022; focusing on the role of ablative fractional lasers and pulsed dye lasers in treating hypertrophic scars, using comprehensive keywords and search syntaxes. Key data extracted included type of scars, the assessment indexes, treatment modalities, side effects and the final conclusion of each article.
View Article and Find Full Text PDFEfficacy of Combined 595-nm Pulsed Dye Laser and Intralesional Corticosteroids Versus Intralesional Corticosteroids Alone for Treating Postmastectomy Hypertrophic Scars and Keloids in Transgender Men: A Randomized Controlled Trial.
J Cosmet Dermatol
March 2025
Division of Dermatology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Background: Top surgery masculinizes the chest appearance for transgender men (TM); however, some individuals may experience hypertrophic scars (HTS) or keloids following the surgery.
Objectives: This study aimed to evaluate the efficacy of combined 595-nm pulsed dye laser (PDL) and intralesional triamcinolone acetonide injection (IL TAC) compared to IL TAC monotherapy for treating HTS and keloids.
Methods: Twenty-five TM with 35 pairs of bilateral symmetric postmastectomy HTS or keloids were randomly allocated to receive the combined PDL and IL TAC on the scar(s) on one side of the chest and IL TAC monotherapy on the contralateral scar(s) in four monthly treatment sessions.
Imaging the Intestinal Transcriptome With Multiplexed Error-Robust Fluorescence In Situ Hybridization (MERFISH).
Curr Protoc
March 2025
Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, Massachusetts.
Multiplexed error-robust fluorescence in situ hybridization (MERFISH) is a massively multiplexed single RNA-molecule imaging technique capable of spatially resolved single-cell transcriptomic profiling of thousands of genes in millions of cells within intact tissue slices. Initially introduced for brain tissues, MERFISH has since been extended to other tissues, where rapid RNA degradation during the preparation process can pose challenges. This protocol outlines the application of MERFISH in one such challenging tissue, the mammalian gastrointestinal tract.
View Article and Find Full Text PDFThe Influence of Bone Density on Stresses in the Periodontal Ligament During Orthodontic Movement-Finite Element Study on Innovative Model.
Materials (Basel)
February 2025
Department of Integrated Dentistry, Wroclaw Medical University, 50-425 Wroclaw, Poland.
Background: Hydrostatic pressure in the periodontal ligament (PDL) plays a critical role in orthodontic treatment, influencing tooth movement and remodeling of periodontal tissue. The relationship between alveolar cortical bone density and the risk of root resorption due to excessive stress in the PDL has not been clearly defined.
Objective: This study aimed to analyze hydrostatic pressure in the periodontal ligament of the tooth roots during en-masse retraction of the maxillary incisors using temporary skeletal anchorage devices (TISADs) after the first premolar extractions, as well as during full arch retraction.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!