Objectives: This study aims to explore whether Connexin 43 (Cx43) in salivary extracellular vesicles (EVs) can serve as a biomarker for diagnosing oral lichen planus (OLP), an inflammatory oral mucosal disorder.
Design: The study assessed disease activity in OLP patients using the reticulum-erosion-ulcer disease activity score. Saliva EVs were isolated and purified with an EV Enrichment Kit. Transmission electron microscopy was used to examine the morphology and size of EVs, while Western blotting verified EV biomarkers. ELISA measured serum inflammatory factors, such as TNF-α and IL-17. The importance of Cx43 in the diagnosis of OLP was evaluated using receiver operating characteristic (ROC) curve analysis, and correlations were determined through Pearson analysis.
Results: The average diameter of isolated saliva EVs was approximately 110 nm, and they expressed high levels of known EV biomarkers. In OLP patients, both Cx43 mRNA and protein levels were significantly higher compared to healthy controls. Furthermore, Cx43 mRNA and protein levels increased gradually with increased disease severity, from the reticular type to the more severe erosion type of OLP. Cx43 mRNA and protein levels were found to effectively diagnosing OLP and correlated significantly with disease activity scores. Moreover, elevated Cx43 mRNA and protein levels showed strong positive correlations with serum TNF-α and IL-17 levels in OLP patients.
Conclusion: Cx43 mRNA and protein levels in salivary EVs serve as effective biomarkers for diagnosing OLP and are significantly associated with disease activity and inflammatory markers. This makes Cx43 a promising candidate for non-invasive diagnosis of OLP.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.archoralbio.2025.106217 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Change and significance of connexin 43 in saliva extracellular vesicles from oral lichen planus patients.
Arch Oral Biol
February 2025
Department of Stomatology, Xuzhou Clinical School of Xuzhou Medical University, Xuzhou, Jiangsu 221000, China; Department of Stomatology, Xuzhou Central Hospital, Xuzhou, Jiangsu 221000, China. Electronic address:
Objectives: This study aims to explore whether Connexin 43 (Cx43) in salivary extracellular vesicles (EVs) can serve as a biomarker for diagnosing oral lichen planus (OLP), an inflammatory oral mucosal disorder.
Design: The study assessed disease activity in OLP patients using the reticulum-erosion-ulcer disease activity score. Saliva EVs were isolated and purified with an EV Enrichment Kit.
Correlation of GD2 Biosynthesis Enzymes With Cancer Stem Cell Markers in Human Breast Cancer.
Cancer Genomics Proteomics
February 2025
The London Breast Institute, Princess Grace Hospital, London, U.K.
Background/aim: The disialoganglioside GD2 has been shown to promote cell proliferation, migration, tumor and metastasis through specific signaling pathways in tumor cells originating from the neuroectoderm, including melanomas, neuroblastomas, glioblastomas, and breast carcinomas. GD2 has therefore emerged as a potential diagnostic biomarker in early malignancy as evidenced by the high specificity of its expression in tumor cells. Furthermore, recent findings show that GD2 might also act as a novel cancer stem cell (CSC) marker.
View Article and Find Full Text PDFConnexin 43 dephosphorylation mediates the Dchs1/YAP/TEAD signaling pathway to induce cardiac fibrosis.
Biochim Biophys Acta Mol Cell Res
March 2025
Center for Cardiovascular Disease, Suzhou Key Laboratory of Cardiovascular Disease, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, Jiangsu 215008, PR China. Electronic address:
Background: The gap junction protein connexin 43 (Cx43) has been implicated in the development of cardiac fibrosis. Our previous findings revealed that Cx43 dephosphorylation at serine 282 (S282) is related to cardiomyocyte apoptosis and arrhythmias in hearts damaged by ischemia/reperfusion. In this study, we investigated the role of Cx43 S282 phosphorylation in cardiac fibrosis.
View Article and Find Full Text PDFAnalysis of key proinflammatory mechanisms in cardiovascular pathology through stimulation with lipopolysaccharide and urban particulate matter in mouse atrial cardiomyocytes.
Environ Toxicol Pharmacol
March 2025
Department of Cardiology, Hospital Clínico Universitario Virgen de la Arrixaca, University of Murcia, Instituto Murciano de Investigación Biosanitaria (IMIB-Arrixaca), CIBERCV, Murcia, Spain. Electronic address:
Air pollution has emerged as one of the leading causes of mortality, aggravating cardiovascular diseases. Urban-particulate matter (PM) can accumulate in the cardiovascular system and through inflammation, trigger systemic damage. One of the key mechanisms of this process could be related to the activation of the inflammasome through the pre-existence of a low-grade endotoxemia and PM presence in the cells.
View Article and Find Full Text PDFSudden cardiac death, arrhythmogenic cardiomyopathy and intercalated disc pathology due to reduced filamin C protein levels: a matter of life and death.
Hum Mol Genet
February 2025
Erich and Hanna Klessmann Institute for Cardiovascular Research and Development, Heart and Diabetes Center North Rhine-Westphalia, Ruhr University of Bochum, Georgstr. 11, 32545 Bad Oeynhausen, Germany.
Mutations in the human FLNC gene encoding filamin C (FLNc) cause a broad spectrum of sporadic and familial cardiomyopathies and myopathies. We report on the genetic, clinical, morphological and biochemical findings in a German family harboring an FLNC variant that leads to severe cardiac disease comprising sudden cardiac death and arrhythmogenic cardiomyopathy. Genetic analysis identified a novel heterozygous FLNC variant in exon 16 (NM_001458.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!