Acetic acid production from corn straw by enzyme catalysis shows its application value in food industry. In this study, a gene encoding for a putative acetyl esterase derived from Sphingobacterium soilsilvae Em02 was discovered in metagenome assembled genome. The gene was expressed in Escherichia coli BL21 to obtain enzyme with a molecular mass of 38.8 kDa. P-Nitrophenyl acetate was used as a substrate to determine the enzyme activity. The enzyme demonstrated optimal activity under conditions of 40 °C and a neutral pH of 7.0. Under optimal conditions, 17.58 mg of acetic acid was obtained using the enzyme from 50 mg corn straw pretreated with amylase. The acetyl esterase derived from Sphingobacterium soilsilvae Em02, demonstrates significant potential for biotechnological applications, particularly in biomass degradation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.enzmictec.2025.110619 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Photoredox Nickel-Catalyzed Coupling/Controllable Defluorination: Access to α-Fluoroarylacetic Amides and Acetates.
Org Lett
March 2025
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, P. R. China.
Herein, an electron donor-acceptor complex (EDA)-enabled photoredox nickel-catalyzed coupling/controllable defluorination domino sequence has been successfully developed, providing an efficient route to a series of α-fluoroarylacetic esters and amides. This methodology accommodates a diverse array of commercially available aryl bromides and chlorodifluoroaryl carboxylic acid derivatives as suitable substrates. Preliminary mechanistic investigations suggest that the reaction is initiated by photoinduced EDA-enabled/nickel-catalyzed direct cross-electrophile coupling, with further defluorination proceeding through the generation of EDA complexes, facilitating a controllable reductive defluorination process.
View Article and Find Full Text PDFExploring dried ginger essential oil as a therapeutic strategy for 5-FU-induced mucositis: gut microbiota and tryptophan metabolite IAA-AHR/IL-22/STAT3 signaling axis.
J Ethnopharmacol
March 2025
State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China; Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China. Electronic address:
Ethnopharmacological Relevance: 5-Fluorouracil (5-FU) commonly induces severe mucositis, causing pain, inflammation, and gastrointestinal dysfunction, which significantly increases patient morbidity and reduces quality of life. In Ayurveda, Traditional Chinese Medicine, and other ethnopharmacological practices, dried ginger has been widely used to alleviate symptoms such as nausea, vomiting, diarrhea, and inflammation, highlighting its important role in traditional medicine.
Aim Of The Study: This study explored the potential of dried ginger essential oil (DGEO) in mitigating intestinal epithelial barrier damage in mice with mucositis induced by 5-FU.
Time dependence of the pre-chromatographic oxidation method for bivalves contaminated with paralytic shellfish poisoning toxins.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
March 2025
Sea and Marine Resources Department (DMRM), The Portuguese Sea and Atmosphere Institute, I.P. (IPMA, IP), Algés, Portugal.
The neurotoxins of the saxitoxin family can be the origin of the human neurological syndrome of paralytic shellfish poisoning via contaminated marine bivalve vectors. A pre-chromatographic oxidation method is the official testing method in the EU, also known as the 'Lawrence method'. It involves several steps, including toxin extraction, solid-phase clean-up, and oxidation to produce fluorescent derivatives.
View Article and Find Full Text PDFRegulating pH distribution of soda saline-alkali soil during electrochemical treatment by conditioning catholyte pH coupled with approaching anodes.
Environ Geochem Health
March 2025
College of GeoExploration Science and Technology, Jilin University, Changchun, 130026, China.
Soda saline-alkali soils pose significant challenges to agricultural productivity due to high pH and excessive sodium content. This study investigated the removal of excess salts in soda saline-alkali soil through electrochemical treatment (ECT). Traditional ECT often led to uneven soil pH distribution, with acidic conditions near the anode and alkaline conditions near the cathode, which limited its effectiveness for soil improvement.
View Article and Find Full Text PDFComparison of 70% and 100% concentration of trichloroacetic acid peeling efficacy with chemical reconstruction of skin scars technique in atrophic acne scar treatment.
Arch Dermatol Res
March 2025
Dermatology Department, Gaziantep University Hospital, Gaziantep, Turkey.
Introduction: The CROSS (Chemical Reconstruction of Skin Scars) technique employs high concentrations of trichloroacetic acid (TCA). This study aimed to compare the efficacy, safety, and tolerability of the CROSS technique using 70% and 100% TCA.
Methods: A total of 40 patients were randomly assigned to two equal groups of 20: the first group received 70% TCA, while the second group was treated with 100% TCA.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!