Background: A newly uncovered parvovirus, Chaphamaparvovirus, continues to be reported across various species. This study investigated the detection and genetic characterization of Chaphamaparvovirus galliform (GaChpV) in poultry, specifically broilers and turkeys, from various regions in Türkiye. To address this, comprehensive sampling and analysis were conducted to better understand the virus's distribution and impact in these avian populations.
Results: In 2023, a total of 1060 fecal samples were collected from 76 broiler flocks (10 healthy and 66 with enteritis) and 30 turkey flocks (10 healthy and 20 with enteritis). Using nested PCR with specific primer sets, the study detected GaChpV in 36 out of 76 broiler flocks (47.3%) and 2 out of 30 turkey flocks (6,6%). Although GaChpV was detected at notable frequencies, the analysis revealed no statistically significant association between GaChpV and enteritis cases (p = 0.617). In this study, the nucleotide sequences (nt) of the capsid genes from GaChpV strains isolated from broilers and turkeys were 99 to 100% identical. Furthermore, these strains exhibited a high degree of genetic similarity ranging from 73 to 98% to Chaphamaparvovirus galliform 2 (GaChpV-2) strains from Europe, China, and Brazil. Complete genome sequencing of a broiler strain (CkChPV/2023/UN-2-TR) yielded a genome of 4,229 nucleotides, with sequence identity ranging from 78.93 to 98.82% compared to other GaChpV strains. Phylogenetic analysis further revealed that the CkChPV/2023/UN-2-TR strain clustered with GaChpV-2 strains, highlighting its genetic relatedness and diversity within the GaChpV family. The study also investigated genetic recombination signals and identified potential B-cell linear epitopes, contributing to a better understanding of the virus's genetic diversity and antigenic characteristics.
Conclusions: This report represents the first detection of GaChpV in turkey and broiler flocks in Türkiye. Notably, research on this topic in turkeys is quite limited. The data derived from this study will contribute to elucidating the molecular epidemiology and evolutionary dynamics of GaChpV.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889929 | PMC |
| http://dx.doi.org/10.1186/s12917-025-04612-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
First detection and molecular characterization of Chaphamaparvovirus galliform in broiler and turkey flocks in Türkiye.
BMC Vet Res
March 2025
Faculty of Veterinery Medicine, Department of Virology, Firat University, Elazig, 23110, Türkiye.
Background: A newly uncovered parvovirus, Chaphamaparvovirus, continues to be reported across various species. This study investigated the detection and genetic characterization of Chaphamaparvovirus galliform (GaChpV) in poultry, specifically broilers and turkeys, from various regions in Türkiye. To address this, comprehensive sampling and analysis were conducted to better understand the virus's distribution and impact in these avian populations.
View Article and Find Full Text PDFDetection of chicken chapparvovirus 2 in chickens with hemorrhagic hepatitis in Japan.
J Vet Med Sci
April 2024
Laboratory of Microbiology, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Article Synopsis
- Chicken chaphamaparvovirus is linked to diarrhea and was found in fecal samples from sick chickens in Japan.
- Affected chickens experienced severe liver hemorrhage, confirmed through necropsy, and showed specific cellular changes under histological analysis.
- High-throughput sequencing and PCR tests confirmed the presence of chicken chapparvovirus 2 in all tested chickens, suggesting it may play a role in causing hemorrhagic hepatitis.
Multienzyme isothermal rapid amplification and lateral flow dipstick combination assay for visible detection of chicken chaphamaparvovirus.
Poult Sci
December 2023
College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China.
Chicken chaphamaparvovirus (CkChpV) is a newly emerging pathogen that is currently prevalent in chickens with diarrhea symptoms. To diagnose CkChpV more conveniently and rapidly, this study established a multienzyme isothermal rapid amplification (MIRA) assay, with a reaction time of only 15 min and optimal reaction temperature of 38°C. In combination with the lateral flow dipstick assay, the CkChpV-MIRA assay can be completed within 20 min.
View Article and Find Full Text PDFMolecular characteristics of novel chaphamaparvovirus identified in chickens.
Poult Sci
March 2023
College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China.
Chicken chaphamaparvovirus (CkChpV) is a novel parvovirus species that belongs to the Chaphamaparvovirus genus and is frequently detected in different vertebrates exhibiting diarrhea symptoms. In this study, screening tests were performed on samples from 478 chickens, including 357 with diarrhea and 121 healthy, collected from 25 farms in China to investigate CkChpV infection in China. CkChpV, avian nephritis virus, rotavirus, chicken parvovirus, Newcastle disease virus, infectious bronchitis virus, chicken proventricular necrosis virus, and chicken circovirus were all detected in the samples at a positivity rate of 32%, 9%, 6%, 2%, 2%, 1%, 0%, and 0%, respectively.
View Article and Find Full Text PDFUnravelling Bile Viromes of Free-Range Laying Chickens Clinically Diagnosed with Spotty Liver Disease: Emergence of Many Novel Chaphamaparvoviruses into Multiple Lineages.
Viruses
November 2022
Department of Microbiology, Anatomy, Physiology and Pharmacology, School of Agriculture, Biomedicine and Environment, La Trobe University, Melbourne, VIC 3086, Australia.
Spotty liver disease (SLD) causes substantial egg production losses and chicken mortality; therefore, it is a disease that concerns Australian egg farmers. Over the last few decades, much research has been conducted to determine the etiologic agents of SLD and to develop potential therapeutics; however, SLD still remains a major issue for the chicken industries globally and remained without the elucidation of potentially multiple pathogens involved. To help fill this gap, this study was aimed at understanding the viral diversity of bile samples from which the SLD-causing bacterium, , has been isolated and characterised.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!