AI Article Synopsis
- Coenzyme Q (CoQ) is a vital compound in energy production within cells but has poor bioavailability when traditionally administered, creating a need for better delivery systems.
- A new multi-step process was established to create and analyze CoQ-loaded nanomicelles, leading to the synthesis of ten different formulations and the use of advanced analytical techniques for precise measurement.
- This novel approach not only allows for improved quality control of these formulations but also addresses the challenges of existing standards in the field, ensuring stability and reproducibility.
Article Abstract
Background: Coenzyme Q (CoQ) is a lipid-soluble compound naturally found in both animals and humans, which plays a key role as an electron carrier in the mitochondrial respiratory chain activity. But, when administered in the form of an oil solution or in water and/or oil suspension or emulsion (traditional way), it presents a poor bioavailability. Hence the need to prepare different encapsulation systems presenting better bioavailability. Analytical strategies are needed to control CoQ bioavailability or not forms in foods and pharmaceutical preparations.
Results: A multi-step approach was developed for the screening of Coenzyme Q-loaded nanomicelles and its subsequent precise analytical determination. Due to the lack of available standards, ten different formulations of Coenzyme Q-loaded micelles were synthesized, and the screening process was carried out over the particle size, bioactive compound encapsulation and components identification by spectroscopic, microscopy and mass spectrometry techniques. Finally, the positive samples for all the screening steps were subjected to a quantitative analysis using LC/MS. The proposed quantification method showed excellent linearity in the concentration ranges of 0.5-10 μg/L (R = 0.9993) for Coenzyme Q and 50.0-7.5·10 μg/L (R = 0.9991) for the selected surfactant Kolliphor® HS15. Additionally, low limits of detection and quantification, along with remarkable accuracy and precision were obtained. Thus, this approach would allow and adequate quality control of these nanomaterials.
Significance: The novelty lies in the synthesis and characterization of distinct CoQ-loaded micelle formulations, characterized using dynamic light scattering (DLS) to determine particle size and polydispersity index, ensuring the stability and reproducibility of the synthesis process. The innovative methodology developed addresses the lack of certified standards and provides a robust framework for the quality control of nanomaterials, significantly advancing the field of analytical nanometrology applied to CoQ available forms.
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| http://dx.doi.org/10.1016/j.aca.2025.343820 | DOI Listing |
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Article Synopsis
- Coenzyme Q (CoQ) is a vital compound in energy production within cells but has poor bioavailability when traditionally administered, creating a need for better delivery systems.
- A new multi-step process was established to create and analyze CoQ-loaded nanomicelles, leading to the synthesis of ten different formulations and the use of advanced analytical techniques for precise measurement.
- This novel approach not only allows for improved quality control of these formulations but also addresses the challenges of existing standards in the field, ensuring stability and reproducibility.
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