In this study, we isolated a novel mycovirus from Lasiodiplodia pseudotheobromae strain YY-1, which we named "Lasiodiplodia pseudotheobromae mycovirus 1" (LpMyV1). The complete genome of LpMyV1 is 2877 bp in length and contains two non-overlapping open reading frames (ORF1 and ORF2). ORF1 encodes a putative protein of 316 amino acids with a molecular weight of 34.1 kDa that shares similarity with the coat proteins of several mycoviruses. ORF2 encodes a protein of 561 amino acids with a molecular weight of 63.2 kDa that contains a conserved RNA-dependent RNA polymerase (RdRp) domain. BLASTp results showed that the RdRp of LpMyV1 shares the highest similarity with those of non-segmented dsRNA viruses. A multiple sequence alignment and phylogenetic analysis indicated that LpMyV1 is a new member of the proposed genus "Unirnavirus". This is the first report of a mycovirus from L. pseudotheobromae.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00705-025-06263-z | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Molecular characterization of a novel non-segmented double-stranded RNA mycovirus isolated from the phytopathogenic fungus Lasiodiplodia pseudotheobromae.
Arch Virol
March 2025
College of Plant Protection, Henan Agricultural University, Zhengzhou, China.
In this study, we isolated a novel mycovirus from Lasiodiplodia pseudotheobromae strain YY-1, which we named "Lasiodiplodia pseudotheobromae mycovirus 1" (LpMyV1). The complete genome of LpMyV1 is 2877 bp in length and contains two non-overlapping open reading frames (ORF1 and ORF2). ORF1 encodes a putative protein of 316 amino acids with a molecular weight of 34.
View Article and Find Full Text PDFAntibacterial Activity, GC MS Analysis and In Silico Validation of Bioactive Compound from Endophytic Fungus Lasiodiplodia pseudotheobromae EF-9.
Chem Biodivers
November 2024
PG & Research Department of Biotechnology, Sri Sankara Arts and Science College, University of Madras, Enathur, Kanchipuram, Tamil Nadu, 631 561, India.
Secondary metabolites synthesized by endophytic fungi have garnered significant interest for their broad applications in treating various ailments. In this study involving 20 plant samples, 11 endophytic fungi were isolated and cultured, and Lasiodiplodia pseudotheobromae EF-9, derived from Hibiscus rosa-sinensis, demonstrated greater antibacterial efficacy than the other isolated endophytes. Phylogenetic analyses using 18S rRNA gene confirmed the EF-9 identity as L.
View Article and Find Full Text PDFpartially overlap on asymptomatic and symptomatic tissues of in agroecosystems and conservation areas in northern South Africa.
Fungal Syst Evol
June 2024
DSI-NRF Centre of Excellence in Plant Health Biotechnology (CPHB), Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa.
Members of the are well-known endophytes and stress-related pathogens. We recently characterised the diversity of in healthy tissues of three tree species in the , namely , and . Here we ask how that diversity compares with the diversity associated with dieback on those tree species.
View Article and Find Full Text PDFFirst report of causing soft rot of plum in China.
Plant Dis
January 2024
Guizhou University, 71206, Guizhou Engineering Research Center of Fruit Crops, College of Agriculture, Guizhou University, Guiyang, Guizhou, China;
Article Synopsis
- - Plum (Prunus salicina) is a significant global fruit species, and in June 2023, symptoms of postharvest soft rot were noted at various fruit markets in Guiyang, China, affecting 20-25% of the plums with severe disease severity.
- - The disease caused visible fruit rot, with symptoms like water-soaked tissue and white fungal growth emerging four days after infection, leading to substantial spoilage.
- - Laboratory investigations involved isolating the fungus responsible, which was identified as Lasiodiplodia pseudotheobromae based on morphological characteristics and DNA analysis, including amplification of several genetic loci.
First Report of Causing Necrosis and Canker Disease on Pear Tree in China.
Plant Dis
January 2024
Huazhong Agricultural University, 47895, College of Plant Science and Technology, Wuhan, Hubei , China;
Article Synopsis
- Pear is an important industry in China, and in March 2023, lesions were found on the trunks of Osmanthus pear trees in a Guangxi orchard.
- A study in August revealed that out of 40 observed trees, 21 had multiple lesions while 19 had fewer lesions; tissue samples were collected for pathogen isolation.
- Six fungal isolates were obtained, with one (GX-3) being characterized and found to match the morphology of *Lasiodiplodia pseudotheobromae*, and its genetic sequences were deposited in NCBI for identification.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!