Here, we describe the applicability of a variety of functionalized magnetic beads (MBs) for DNA extraction under different lysis conditions. Various MBs, including hydroxyl, carboxylic, amino, chitosan-modified, and streptomycin-modified MBs (MBs-STR), were compared based on their extracted DNA quantity and quality. The results presented that the MBs exhibited different DNA adsorption capacities under specific conditions. Notably, MBs-STR maintained stable DNA adsorption capacity upon different conditions through a non-intercalative binding mechanism between STR and DNA minor groove. This discovery advances antibiotic-based DNA extraction strategies, offering a flexible, efficient alternative for biomedical applications while enriching the toolkit for nucleic acid isolation.
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| http://dx.doi.org/10.1016/j.ab.2025.115836 | DOI Listing |
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