We used scanning electron microscopy to visualize the regeneration of goldfish scales on day 3 in vivo. Several vesicle-like structures of 100-700 nm diameter flowed onto the fibrous sheets in groups of spindle-shaped bodies arranged in the same direction. Transmission electron microscopy revealed that these structures were encircled by the lipid bilayer membrane. In addition, some had a small mass of high electron density. Scanning electron microscopic observations of specimens treated with bleach revealed particles of almost the same size as the observed electron-dense mass scattered between fibers, with a thickness of approximately 50 nm on day 3 of scale regeneration. The diameter of these particles increased by 5 times on day 14, sticking closely to the fibers. Furthermore, elemental analysis using electron probe microscopy showed that the particles were composed of calcium and phosphorous. These results confirmed that the spindle-shaped bodies and vesicle-like structures were osteoblasts and matrix vesicles, respectively.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00795-025-00427-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Efficacy and safety of platelet-rich plasma as an adjunct therapy to split thickness skin graft in burn patients with granulating raw wounds: a prospective, randomized, double-blind study-study protocol.
Trials
March 2025
Department of Burn and Plastic Surgery, Govt. Kilpauk Medical College, Chennai, Tamil Nadu, India.
Background: Burn wounds are commonly encountered in clinical settings and the management aims at the prevention of mortality and morbidity due to disability. The platelet-rich plasma (PRP) is blood-derived biomaterial that is enriched with growth factors and cytokines that facilitate wound healing. The PRP has proven its efficacy in various other wounds, but its role in post-burn raw areas and graft take has not been validated.
View Article and Find Full Text PDFPurpose: The aim of this study was to compare the efficacy, clinical outcomes, and complications of cable-asisted bone transport (CASt) and circular external fixator-assisted bone transport (CEFt) methods in the management of bone defects of the tibia.
Methods: A retrospective analysis was conducted on 32 patients who underwent segmental bone transport for tibial bone defects between January 2006 and January 2020 and met the study inclusion criteria. Patients were categorized into two groups: CASt group (n = 16) and CEFt group (n = 16).
Mesenchymal stem cells therapy for the treatment of non-union fractures: a systematic review and meta-analysis.
BMC Musculoskelet Disord
March 2025
Department of Joint Surgery, Central Hospital Affiliated to Shandong First Medical University, NO. 105, Jiefang Road, Jinan, 250013, China.
Background: This meta-analysis aimed to pool the existing evidence to determine the clinical efficacy and safety of mesenchymal stem cells (MSC) in patients with non-unions.
Methods: A systematic search in PubMed and Scopus was performed until October 2024 to gather pertinent studies. The inclusion criteria included participants with non-unions, the intervention of MSC administration, a comparator of standard treatment (bone graft), and outcomes focused on healing rate, healing time, or side effects.
Generating New Meniscus Therapies via Recent Breakthroughs in Development, Model Systems, and Clinical Diagnostics.
J Orthop Res
March 2025
Division of Sports Medicine, Connecticut Children's Medical Center, Farmington, Connecticut, USA.
Over 850,000 surgeries are performed to treat meniscal injuries each year in the United States. Even with repair, patients are likely to develop osteoarthritis (OA) within the next two decades. There is a pressing clinical need to improve meniscal repair procedures to restore tissue function and prevent joint degeneration later in life.
View Article and Find Full Text PDFNanofibrous Chitin Clusters Constructed via Controllable Crystalline Structure Transition for 3D Functional Materials.
Small
March 2025
Interdisciplinary Institute of NMR and Molecular Sciences, School of Chemistry and Chemical Engineering, Hubei Province for Coal Conversion and New Carbon Materials, Wuhan University of Science and Technology, Wuhan, 430081, P. R. China.
Intensively studied polymeric particle production technologies often rely on the combination of polymer self-assembly and particle processing techniques. Herein, an elegant crystallization transition-mediated strategy is proposed to confine molecular self-assembly within a limited range, avoiding the need for extra particle processing steps. This approach enables the production of the regenerated nanofibrous chitin clusters woven with the helical nanofibers.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!