Raw milk is known to harbor a complex microbial community, including microorganisms of technological and human health interest. However, it can also be a source of pathogenic and spoilage bacteria, such as spore-forming bacteria and Pseudomonas spp. Despite cleaning and disinfection procedures, biofilms in milking machines are difficult to remove and represent a major source of milk contamination. This work aims to describe biofilms in milking machines at both the laboratory and farm scales. Encouraging studies on the microbiota of milking machine biofilms, the parameters influencing changes in biofilm composition, and the methods used to characterize them are essential for managing the formation and composition of these biofilms. Enhancing such knowledge will help improve the understanding of milking machine biofilms and their impact on the quality of milk and dairy products.
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http://dx.doi.org/10.3168/jds.2024-25352 | DOI Listing |
Microb Pathog
March 2025
Molecular Biology and Bioinformatics Laboratory, Department of Gynecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur 1706, Bangladesh. Electronic address:
Pseudomonas putida is one of the emerging pathogens responsible causing mastitis in lactating animals. This study investigated the prevalence, antimicrobial resistance (AMR), genetic diversity and virulence factor genes (VFGs) to highlight the pathogenic potentials of P. putida strains isolated from milk, feces and farm soil of bovine clinical mastitis (CM).
View Article and Find Full Text PDFJ Dairy Sci
March 2025
Institut de l'Elevage, 75595 Paris Cedex 12, France. Electronic address:
Raw milk is known to harbor a complex microbial community, including microorganisms of technological and human health interest. However, it can also be a source of pathogenic and spoilage bacteria, such as spore-forming bacteria and Pseudomonas spp. Despite cleaning and disinfection procedures, biofilms in milking machines are difficult to remove and represent a major source of milk contamination.
View Article and Find Full Text PDFInt J Food Microbiol
February 2025
Institute of Human Nutrition, Department of Food Hygiene and Quality Management, Warsaw University of Life Sciences-SGGW, 02-786 Warsaw, Poland.
Cronobacter sakazakii and Enterobacter cloacae exhibit the ability to form biofilms, making them resistant to drying, antibiotics, and changes in pH. These biofilms can adhere to different surfaces, including tissues, catheters, enteral feeding tubes, and work surfaces, potentially leading to cross-infection risks in both the food and medical sectors. The objective of this study was to develop a predictive model of biofilm formation over time by C.
View Article and Find Full Text PDFNat Prod Rep
March 2025
Department of Paleobiotechnology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, 07745 Jena, Germany.
Covering: 2000 up to the first half of 2024Milk and its derived dairy products have long been integral to the human diet, with evidence of consumption dating back over 9000 years. Milk's high nutritional value renders dairy products an important element of human diet while also offering a fertile environment for microbial growth. Beneficial microorganisms in dairy products are often associated with biogenic and probiotic effects, whereas spoilage or pathogenic microorganisms can pose health risks.
View Article and Find Full Text PDFPharmaceutics
February 2025
Centro de Investigación Austral Biotech, Facultad de Ciencias, Universidad Santo Tomás, Avenida Ejército 146, Santiago 8370003, Chile.
Background/objectives: Bovine mastitis (BM), a prevalent and economically burdensome bacterial infection affecting dairy cattle, poses a significant challenge to the dairy industry. The traditional approach to combating BM, relying heavily on antibiotics, faces growing concerns due to the increasing antibiotic resistance exhibited by pathogens. The objective of this study was to evaluate and determine the antimicrobial and anti-biofilm potential of chitosan nanoparticles (NQo) on strains isolated from milk samples obtained from dairy areas in southern Chile from cows diagnosed with BM.
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