Toxicity enhancement of microplastics released from food containers through thermal aging: Absorbing more serum proteins thus activating the innate immune response via actin polymerization.

This study examined the effects of hot high-fat simulants on the physicochemical properties of microplastics (MPs) from polypropylene (PP)-, low-density polyethylene (LDPE)-, and polylactic acid (PLA)-based single-use food container (SUFC) leachates and those of aging on their immunomodulatory effectors. Scenario studies have demonstrated that MPs were released from these three types of SUFCs. LDPE- and PLA-based SUFCs also released cellulose. Among the SUFCs, only the PP leachates particles exhibited a new absorption peak at 1725 cm, which aging phenomenon may be attributed to the presence of unstable tertiary carbon atoms. Subsequently, we investigated the immunomodulatory effects of removing additive both PP and thermal-aged PP with polystyrene (PS) and carboxyl-modified PS (PS-COOH) polymer backbones as reference materials. The findings indicated that thermal-aged PP and PS-COOH induced comparable innate immune responses, with PS-COOH particles exhibiting a similar size to SUFC percolates. Consequently, PS and PS-COOH were selected as original and thermal-aged MPs, respectively, to evaluate the effects of aging on innate immunity. The results revealed thata protein corona formed on both particle types, with more protein adsorption observed on PS-COOH particles. The complex enhanced the phagocytosis of RAW264.7 macrophages and increased the expression of pro-inflammatory genes NOS2 and TNF-α through an actin polymerization cross-linking mechanism. In this study, we investigated how thermal-aged MPs affect innate immune responses using PS-COOH as a model system, emphasizing the importance of a comprehensive safety evaluations of MPs.