The aim of the present study was to test the effects of different doses of anhydrous theacrine (3 and 6 mg.kg-1 of body mass) on 4-km cycling time trial performance. Nineteen (11 men and 8 women) cyclists (30.8 ± 10.0 years, 71.7 ± 11.8 kg, 170.1 ± 9.0 cm, and 17.0 ± 6.2 % of body fat) completed three 4-km cycling time trials after ingesting either placebo, or 3 mg.kg-1 or 6 mg.kg-1 of body mass of theacrine. No significant differences (p < 0.05) were observed in time to cover the 4-km cycling time trial between the placebo, 3 mg.kg-1 of theacrine, and 6 mg.kg-1 of theacrine conditions. However, the intake of theacrine at the dose of 6 mg.kg-1 increased resting systolic and diastolic blood pressure (p < 0.05). Some side effects, such as gastrointestinal discomfort, perception of tachycardia, dizziness, headache, head pressure, hand tremor, and lack of disposition were reported after the ingestion of either 3 or 6 mg.kg-1 of body mass of theacrine. In conclusion, theacrine supplementation does not improve endurance performance and provokes side effects that might restrict its use as a supplement.
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