The diagnosis of chronic Chagas disease (CD) is challenging due to the wide genetic diversity of Trypanosoma cruzi (T. cruzi), the causative agent of CD, and low levels of parasitemia, resulting in low sensitivity and accuracy using existing diagnostics. We report a magneto-immunoassay that employs dually-labeled magnetic beads (DMBs) incorporating a recombinant Tc24 antigen, which is homologous across multiple discrete typing units (DTUs) of T. cruzi. In this pilot study, 102 serum samples from seven endemic countries were tested using this magneto-immunoassay, revealing its ability to distinguish Chagas-positive from Chagas-negative cases more accurately and faster than a standard Tc24-based immunoassay.
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