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Disruption of the miR396 binding site in GROWTH-REGULATING FACTOR 4 enhances grain size in wheat.
Sci China Life Sci
March 2025
Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Blocking miR396 activity by overexpression MIM396 improved switchgrass tiller number and biomass yield.
Biotechnol Biofuels Bioprod
May 2024
College of Grassland Science and Technology, China Agricultural University, Beijing, 100193, China.
Article Synopsis
- MicroRNA396 (miR396) regulates plant growth by repressing growth-regulating factor (GRF) genes, and blocking its expression may enhance tillering and biomass yield in switchgrass.
- Transgenic switchgrass plants with a mimic (MIM396) showed increased tiller numbers and dry weight, despite reduced enzymatic efficiency, due to higher overall biomass.
- The study suggests that MIM396 can be a valuable tool for improving both tiller number and biomass yield in switchgrass by influencing gene expression related to bud growth and development.
Derepression of specific miRNA-target genes in rice using CRISPR/Cas9.
J Exp Bot
October 2021
Institute of Biotechnology, Fujian Academy of Agricultural Sciences/Fujian Provincial Key Laboratory of Genetic Engineering for Agriculture, Fuzhou, China.
MicroRNAs (miRNAs) target specific mRNA molecules based on sequence complementarity for their degradation or repression of translation, thereby regulating various developmental and physiological processes in eukaryotic organisms. Expressing the target mimicry (MIM) and short tandem target mimicry (STTM) can block endogenous activity of mature miRNAs and eliminate the inhibition of their target genes, resulting in phenotypic changes due to higher expression of the target genes. Here, we report a strategy to achieve derepression of interested miRNA-target genes through CRISPR/Cas9-based generation of in-frame mutants within the miRNA-complementary sequence of the target gene.
View Article and Find Full Text PDFDisruption of and improves rice yield under nitrogen-deficient conditions.
Natl Sci Rev
January 2020
Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China.
The microRNA miR396 directly represses () and has been implicated in regulating rice yield and in nitrogen assimilation. Overexpressing the miR396 targets and improves rice yield via increased grain size and panicle branching, respectively. Here, we used CRISPR/Cas9 to assess the function of miR396 genes in rice.
View Article and Find Full Text PDFA missense mutation in Large Grain Size 1 increases grain size and enhances cold tolerance in rice.
J Exp Bot
August 2019
School of Life Sciences, Xiamen University, Xiamen, China.
Grain shape is controlled by quantitative trait loci (QTLs) in rice (Oryza sativa L.). A rice mutant (JF178) with long and large grains has been used in a breeding program for over a decade, but its genetic basis has been unclear.
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