Fused Deposition Modeling (FDM) is a prominent additive manufacturing technique known for its ability to provide cost-effective and fast printing solutions. FDM enables the production of computer-aided 3D designs as solid objects at macro scales with high-precision alignment while sacrificing excellent surface smoothness compared to other 3D printing techniques such as SLA (Stereolithography) and SLS (Selective Laser Sintering). Electro-Spinning (ES) is another technique for producing soft-structured nonwoven micro-scale materials, such as nanofibers. However, compared to the FDM technique, it has limited accuracy and sensitivity regarding high-precision alignment. The need for high-precision alignment of micro-scaled soft structures during the printing process raises the question of whether FDM and ES techniques can be combined. Today, the printing technique with such capability is called Melt Electro Writing (MEW), and in practice, it refers to the basic working principle on which bio-printers are based. This paper aims to examine how these two techniques can be combined affordably. Comparatively, it presents output production processes, design components, parameters, and materials used in output production. It discusses the limitations and advantages of such a hybrid platform, specifically from the perspective of engineering design and its biomedical applications.
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http://dx.doi.org/10.3390/mi16020226 | DOI Listing |
Nat Commun
March 2025
Department of Electrical Engineering, City University of Hong Kong, Kowloon, Hong Kong SAR, China.
Metasurface-enabled optical analog differentiation has garnered significant attention due to its inherent capacity of parallel operation, compactness, and low power consumption. Most previous works focused on the first- and second-order operations, while several significant works have also achieved higher-order differentiation in both real space and k-space. However, how to construct the desired optical transfer function in a practical system to realize scalable and multi-order-parallel high-order differentiation of images in real space, and particularly how to leverage it to tackle practical problems, have not been fully explored.
View Article and Find Full Text PDFNano Lett
March 2025
School of Electronic Science and Engineering/National Laboratory of Solid-State Microstructures, Nanjing University, Nanjing, 210023, P. R. China.
Real-time and precise evaluation of human body temperature offers crucial insights for health monitoring and disease diagnosis, while integration of high-performance and miniaturized sensors remains a challenge. Inspired by the thermal sensory pathway of skin, here we developed a new route for scalable fabrication of rapid-response and miniaturized thermoreceptor sensors using self-aligned in-plane silicon nanowire (SiNW) arrays as sensitive channels. These SiNW arrays, with a diameter of 100 ± 14 nm, were integrated into temperature sensors with a density of 445 devices/cm without using any high-precision lithography.
View Article and Find Full Text PDFClustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated proteins (Cas) systems have revolutionized genome editing by providing high precision and versatility. However, most genome editing applications rely on a limited number of well-characterized Cas9 and Cas12 variants, constraining the potential for broader genome engineering applications. In this study, we extensively explored Cas9 and Cas12 proteins and developed CasGen, a novel transformer-based deep generative model with margin-based latent space regularization to enhance the quality of newly generative Cas9 and Cas12 proteins.
View Article and Find Full Text PDFMicromachines (Basel)
February 2025
Department of Biomedical Engineering, Engineering Faculty, Ahmet Necdet Sezer Campus, Afyon Kocatepe University, Afyonkarahisar 03200, Turkey.
Fused Deposition Modeling (FDM) is a prominent additive manufacturing technique known for its ability to provide cost-effective and fast printing solutions. FDM enables the production of computer-aided 3D designs as solid objects at macro scales with high-precision alignment while sacrificing excellent surface smoothness compared to other 3D printing techniques such as SLA (Stereolithography) and SLS (Selective Laser Sintering). Electro-Spinning (ES) is another technique for producing soft-structured nonwoven micro-scale materials, such as nanofibers.
View Article and Find Full Text PDFNat Commun
March 2025
College of Marine Life Sciences, Ocean University of China, Qingdao, China.
The rapid growth in the number of newly identified DNA and RNA viral sequences underscores the need for an accurate and comprehensive classification system for all viral realms at different taxonomic levels. Here, we establish the Viral Taxonomic Assignment Pipeline (VITAP), which addresses classification challenges by integrating alignment-based techniques with graphs, offering high precision in classifying both DNA and RNA viral sequences and providing confidence level for each taxonomic unit. This tool automatically updates its database in sync with the latest references from the International Committee on Taxonomy of Viruses (ICTV), efficiently classifying viral sequences as short as 1,000 base pairs to genus level.
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