Objectives: The rapid plasma reagin (RPR) test, a traditional method for diagnosing syphilis and evaluating treatment efficacy, relies on subjective interpretation and requires high technical proficiency. This study aimed to develop and validate a user-friendly RPR-artificial intelligence (AI) interpretative tool.
Methods: A dataset comprising 600 images of photographed RPR cards from 276 negative and 223 positive RPR samples was used for model development. The reference result was based on consistent interpretations by at least two out of three experienced laboratory personnel. Then an interpretative model was developed using deep learning algorithms and loaded into smartphones for on-site interpretation at two clinical centers from October 2023 to April 2024.
Results: The model demonstrated an accuracy of 82·67% (95% CI 71·82%-90·09%) for reactive circles and 84·44% (95% CI 69·94%-93·01%) for non-reactive circles. In the field study, 669 specimens showed a sensitivity of 94·85% (95% CI 89·29%-97·73%), specificity of 91·56% (95% CI 88·78%-93·71%), and concordance of 92·23% (95% CI 89·87%-94·09%). The positive predictive value was 74·14% (95% CI 66·86%-80·33%) and negative predictive value was 98.59% (95% CI 96·98%-99·38%).
Conclusions: The tool assists in RPR interpretation standardization, enabling data traceability, and quality control for remote and underdeveloped areas.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jinf.2025.106454 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Maternal supplementation with α-tocopherol inhibits the development of offspring food allergy, H1R signaling and ultimately anaphylaxis early in life.
J Immunol
February 2025
Herman B Wells Center for Pediatric Research, Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN, United States.
Food allergy has had a rapid rise in prevalence, and thus it is important to identify approaches to limit the development of food allergy early in life. Because maternal dietary supplementation with α-tocopherol (α-T), an isoform of vitamin E, during pregnancy and nursing increases neonate plasma levels of α-T and can limit neonate development of other allergies, we hypothesized that α-T can limit development of food allergy. To assess this, male mice with mutations in their skin barrier genes (FT-/- mice) were mated with wild-type females that received a diet supplemented with α-tocopherol or a control diet.
View Article and Find Full Text PDFEnhanced packaging of U6 small nuclear RNA and splicing-related proteins into extracellular vesicles during HIV infection.
Sci Adv
March 2025
Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
U6 small nuclear RNA (U6 snRNA), a critical spliceosome component primarily found in the nucleus, plays a vital role in RNA splicing. Our previous study, using the simian immunodeficiency virus (SIV) macaque model, revealed an increase of U6 snRNA in plasma extracellular vesicles (EVs) in acute retroviral infection. Given the limited understanding of U6 snRNA dynamics across cells and EVs, particularly in SIV infection, this research explores U6 snRNA trafficking and its association with splicing proteins in the nucleus, cytoplasm, and EVs.
View Article and Find Full Text PDFDevelopment of colloidal gold test strip based on the BsVg819 gene fragment of vitellogenin of Bostrichthys sinensis for the detection of vitellogenin in multiple fish species.
Fish Physiol Biochem
March 2025
Key Laboratory of the Coastal and Wetland Ecosystems, Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen, 361102, Fujian, China.
As an environmental estrogen biomarker, the yolk precursor, vitellogenin (Vtg) is widely used in the assessment of estrogen pollution in aquatic environment. Currently, the detection of Vtg in plasma is mainly achieved by enzyme-linked immunosorbent assay (ELISA) method based on Vtg antibodies. However, due to differences in the immunological epitopes of Vtg from various species, Vtg antibodies have low universality.
View Article and Find Full Text PDFCriteria and prognostic factors for survival and success rates for autotransplantation in the area of first and second molars.
Swiss Dent J
March 2025
Riga Stradiņš University, Faculty of Dentistry.
Autotransplantation of immature third molars is an effective treatment option, offering high success rates, minimal complications, and notable improvements in occlusal function and dental aesthetics. This study aims to review and evaluate the available evidence specifically on the survival and success rates of immature autotransplanted third molars and to identify prognostic factors that influence these outcomes. A comprehensive search was conducted in the Elsevier Journals (ScienceDirect), MEDLINE (PubMed), and Dentistry & Oral Sciences Source (EBSCOhost) databases up to May 2024.
View Article and Find Full Text PDFDevelopment and Validation of a HPLC-MS/MS Method for the Determination of Compound 13b in Rat Plasma and Its Application to a Pharmacokinetic Study.
Biomed Chromatogr
April 2025
NMPA Key Laboratory for Research and Evaluation of Drug Metabolism & Guangdong Provincial Key Laboratory of New Drug Screening & Guangdong-Hongkong-Macao Joint Laboratory for New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China.
Compound 13b, a newly identified anthraquinone derivative, has demonstrated potent efficacy against the Zika virus. To explore the bioavailability and pharmacokinetic properties of compound 13b, a robust and sensitive HPLC-MS/MS method was established and verified to determine its plasma concentration in rats. Sample preparation involved protein precipitation using acetonitrile with testosterone as an internal standard.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!