Bacillus subtilis (B. subtilis) is a crucial industrial microorganism for lignocellulose biomass degradation. However, wild-type strains from natural environments have inherent deficiencies in the composition of cellulase genes, so constructing recombinant strains through genome engineering is a generalizable strategy to overcome these shortcomings. Herein, eglS, cel48S, and bglS were integrated into the aprE, epr, and amyE loci of the B. subtilis RLI2019 chromosome, respectively, through CRISPR/Cas9-mediated genome editing, deriving the engineered strain B. subtilis AEA3. The activities of endoglucanase, exoglucanase, β-glucosidase, xylanase, and total cellulase in B. subtilis AEA3 were enhanced by 3.1-fold, 6.6-fold, 3.0-fold, 1.2-fold, and 1.8-fold, respectively, reaching 26.31 U/mL, 9.77 U/mL, 3.91 U/mL, 19.63 U/mL, and 2.42 U/mL. Notably, the engineered strain improved the saccharification efficiency of crop straws, effectively disrupting fiber structure, and significantly reducing the content of neutral and acid detergent fibers, lignocellulose and hemicellulose. In summary, this study provides a general strategy for enhancing the cellulose degradation capabilities of B. subtilis through comprehensive and systematic multi-module genetic engineering, broadening its potential application in lignocellulose biomass conversion.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijbiomac.2025.141727 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exploring the Exclusive Isolation of Pseudomonas syringae in Peltigera Lichens via Metabolite Analysis and Growth Assays.
Environ Microbiol
March 2025
School of Biosciences and Birmingham Institute of Forest Research, University of Birmingham, Birmingham, UK.
The specific association of the potentially plant-pathogenic Pseudomonas syringae with Peltigera lichens raises questions about the factors driving this host specificity. To explore this, the metabolic profile of seven lichen species belonging to three genera (Cladonia, Peltigera and Stereocaulon) was analysed using LC-MSMS. In addition, we assessed the growth of P.
View Article and Find Full Text PDFActivation of gut microbiota-hif1α axis effectively restores resistance to Aeromonas veronii caused by improper administration of AiiO-AIO6.
J Nutr
March 2025
China-Norway Joint Lab on Fish Gut Microbiota, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. Electronic address:
Background: Feeding adult zebrafish a diet supplemented with quenching enzyme AiiO-AIO6 for 3 wk improved the growth performance and disease resistance. However, when the feeding period was extended to 8 wk, zebrafish's disease resistance to Aeromonas veronii decreased.
Objective: We investigated the mechanisms of the reduced disease resistance of zebrafish induced by feeding on AiiO-AIO6 supplemented diet for a long-term (8 wk), and assessed the effectiveness of feed additives in restoring the low disease resistance.
AprMH1 subtilisin from Bacillus zhangzhouensis MH1: Molecular cloning, characterization, and homology modeling.
Int J Biol Macromol
March 2025
University of Jeddah, Applied College, Biology Department, Jeddah, Saudi Arabia.
The growing demand for robust proteases in industrial applications, particularly those based on the widely used subtilisin family, necessitates the development of novel and improved enzymes. This study reports exploration and characterization of a subtilisin, AprMH1, isolated from a recently identified Bacillus zhangzhouensis MH1 (NCBI Acct. No.
View Article and Find Full Text PDFEffective microbial formulations using sustainable carriers for the remediation of plastic-affected soils.
J Environ Manage
March 2025
Department of Biology and Geology, CITE II-B, University of Almeria, Agrifood Campus of International Excellence, ceiA3, CIAIMBITAL, ctra. Sacramento s/n, 04120, Almeria, Spain.
The increasing use of mulching films in intensive agriculture, together with their inefficient end-of-life disposal, has led to a significant plastic accumulation in soils, which contributes to disrupting ecosystems. The aim of this work was to determine the ability of different sustainable carriers to harbor and introduce plastic-degrading microorganisms into contaminated soils to provide a biotechnological tool that potentially enhances plastic decontamination, ameliorating the harmful effect of this type of pollutant in soil. To this end, pure cultures and co-cultures of Bacillus subtilis and Pseudomonas alloputida (specialized plastic-degrading strains) were added to three sustainable carriers (vermicompost, biochar, and calcium alginate beads) for the preparation of microbial formulations.
View Article and Find Full Text PDFAcronyculatin S, a polyphenolic isoprenylated acetophenone benzofuran with antibacterial properties from the flowers of .
Nat Prod Res
March 2025
Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand.
Phytochemical investigation of the flowers of led to the isolation of eight compounds. They consist of one undescribed polyphenolic isoprenylated acetophenone benzofuran, acronyculatin S (), and seven known compounds: demethylacrovestone (), acrovestone (), acroflavone A (), 3,5,4'-trihydroxy-3',8-dimethoxy-7-(3-methylbut-2-enyloxy)flavone (), xanthotoxin (), isopimpinellin () and -methylflindersine (). The structures of the isolated compounds were elucidated by thorough analysis of spectroscopic (IR, NMR and ECD) and spectrometric (MS) data.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!