Studying zebrafish embryos' growth through imaging them in their natural growth environment may reveal what has not been possible through the current imaging technique which uses mechanically-confining and nutrient-limiting gel, like agarose. This paper presents, for the first time, the imaging of live zebrafish embryos in their natural environment over 20 hours through acoustic tweezers capable of contactless trapping and precise manipulation via trapping without standing waves. The tweezers is shown to trap and hold a zebrafish embryo in its growth medium from 17 hours post fertilization (hpf) to 37 hpf under a Light-Sheet microscope for imaging. The continuous trapping and imaging reveal organ development, such as the tail, eyes, ears, and pigmentation. The method is safe, as evidenced by natural development, heartbeats, and tail movement.
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http://dx.doi.org/10.1109/TBME.2025.3548557 | DOI Listing |
Ultrasonics
March 2025
Key Laboratory of Biomedical Imaging Science and System, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, PR China. Electronic address:
Cells' ability to sense and respond to mechanical stimuli is fundamental to various biological processes and serves as a crucial biomarker of their physiological and pathological states. Traditional methods for assessing cell mechanical properties, such as atomic force microscopy and micropipette aspiration, are hindered by complex procedures and the risk of cellular damage due to direct contact. Here we introduce a novel non-contact acoustic squeezer that leverages focused interdigital transducers to induce cell deformation through a robust standing surface acoustic wave (SSAW) field.
View Article and Find Full Text PDFSoft Matter
March 2025
Huygens-Kamerlingh Onnes Laboratory, Leiden University, P.O. Box 9504, 2300 RA Leiden, The Netherlands.
Catalytic microswimmers typically swim close to walls due to hydrodynamic and/or phoretic effects. The walls in turn are known to affect their propulsion, making it difficult to single out the contributions that stem from particle-based catalytic propulsion only, thereby preventing an understanding of the propulsion mechanism. Here, we use acoustic tweezers to lift catalytically active Janus spheres away from the wall to study their motion in bulk and when approaching a wall.
View Article and Find Full Text PDFStudying zebrafish embryos' growth through imaging them in their natural growth environment may reveal what has not been possible through the current imaging technique which uses mechanically-confining and nutrient-limiting gel, like agarose. This paper presents, for the first time, the imaging of live zebrafish embryos in their natural environment over 20 hours through acoustic tweezers capable of contactless trapping and precise manipulation via trapping without standing waves. The tweezers is shown to trap and hold a zebrafish embryo in its growth medium from 17 hours post fertilization (hpf) to 37 hpf under a Light-Sheet microscope for imaging.
View Article and Find Full Text PDFIEEE Trans Ultrason Ferroelectr Freq Control
February 2025
Holographic acoustic tweezers have various biomedical applications due to their ability to flexibly and rapidly synthesize acoustic fields for manipulating single or multiple particles. Existing multi-particle manipulation techniques are usually realized by precisely designing the incident wave's phase distribution to synthesize a complex and steady-state acoustic field containing multiple acoustic trapping beams. However, interference effects between multiple beams tend to produce artifacts that trap particles in unwanted positions, limiting accuracy and the number of manipulated particles.
View Article and Find Full Text PDFLight Sci Appl
February 2025
Institute of Precision Optical Engineering, School of Physics Science and Engineering, Tongji University, Shanghai, 200092, China.
Optical sorting combines optical tweezers with diverse techniques, including optical spectrum, artificial intelligence (AI) and immunoassay, to endow unprecedented capabilities in particle sorting. In comparison to other methods such as microfluidics, acoustics and electrophoresis, optical sorting offers appreciable advantages in nanoscale precision, high resolution, non-invasiveness, and is becoming increasingly indispensable in fields of biophysics, chemistry, and materials science. This review aims to offer a comprehensive overview of the history, development, and perspectives of various optical sorting techniques, categorised as passive and active sorting methods.
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