Resistome and virulome determination in using next-generation sequencing with target-enrichment technology.

The identification of infection from gastric biopsy samples requires PCR or bacterial cultures. However, it is difficult to culture because it is a fragile bacterium. Next-generation sequencing (NGS) allows direct assessment of the resistome and virulome. Here we describe a new NGS method for studying the resistome and virulome of directly from gastric biopsies, based on enrichment analyses and targeted sequencing of DNA. In all, 19 DNA samples from human gastric biopsies that tested positive for were analyzed. The Agilent SureSelectXT target-enrichment protocol was used with a custom bait library prior to sequencing using the Agilent MagnisDx NGS Library Prep System. NGS sequencing was performed on the Illumina iSeq 100 sequencer using RNA probes for virulence, resistance, and molecular typing genes. The method yielded significant results with a limit of detection of around 1.8e CFU per mL . Mutations in the sequence associated with macrolide resistance and in the quinolone resistance-determining region of associated with levofloxacin resistance were correctly identified. The results of MLST phylogeny analyses performed after target-enrichment were consistent with those obtained via conventional Sanger sequencing. Among the -positive isolates, the gene was detected correctly, and the genotype was determined. In conclusion, our enrichment method enables rapid assessment of the resistome and virulome of directly from fresh gastric biopsies.IMPORTANCE, a bacterium that infects at least 50% of the world population, is often treated by probabilistic antimicrobial therapies due to the lack of antimicrobial resistance data provided by clinical laboratories to clinicians. However, targeted antimicrobial therapies are increasingly recommended to achieve efficient eradication with a limited impact on the gut microbiota and with fewer adverse events for the patient. Recent advancements in next-generation sequencing strategies have opened new opportunities in the diagnosis of infection. The significance of our research is the development of a novel next-generation sequencing strategy based on target-enrichment. This approach enables the identification of the resistome and the virulome of directly from gastric biopsies, providing clinicians with a broad overview of therapeutic options.