Automatic detection and identification of pharynxgolaryngeal activities (PLA) such as swallowing, speaking, and coughing, are crucial steps in developing a system for diagnosing dysphagia (swallowing disorders) using high-resolution cervical auscultation (HRCA) signals. Using sensors placed on the throat, HRCA provides clinicians with a non-invasive means of assessing swallowing. This study introduces a new approach based on depthwise CNN and LSTM networks to automatically detect and identify PLA in synchronized accelerometer and microphone signals. This method achieves an F of 88% for swallow detection across seven hours of HRCA signals recorded from 42 healthy subjects under both supervised and ecological conditions. It outperforms state-of-the-art systems in real-world settings. On other activities, the system reaches an F of 93% for phonation and 86% for airway defense mechanisms, making it a promising tool to assist clinicians in the analysis of HRCA signals and a reliable base towards non-invasive assessment of dysphagia.
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http://dx.doi.org/10.1109/EMBC53108.2024.10782007 | DOI Listing |
Annu Int Conf IEEE Eng Med Biol Soc
July 2024
Automatic detection and identification of pharynxgolaryngeal activities (PLA) such as swallowing, speaking, and coughing, are crucial steps in developing a system for diagnosing dysphagia (swallowing disorders) using high-resolution cervical auscultation (HRCA) signals. Using sensors placed on the throat, HRCA provides clinicians with a non-invasive means of assessing swallowing. This study introduces a new approach based on depthwise CNN and LSTM networks to automatically detect and identify PLA in synchronized accelerometer and microphone signals.
View Article and Find Full Text PDFAnnu Int Conf IEEE Eng Med Biol Soc
July 2024
Swallowing assessment is a crucial task to reveal swallowing abnormalities. There are multiple modalities to analyze swallowing kinematics, such as videofluoroscopic swallow studies (VFSS), which is the gold standard method, and high-resolution cervical auscultation (HRCA), which is a noninvasive technique that uses a triaxial accelerometer attached to the patient's neck. Deep learning models play an essential role in data driven analysis of swallowing landmarks using VFSS and/or HRCA as input data.
View Article and Find Full Text PDFAnal Chem
March 2025
College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian 350116, China.
Herein, we present an innovative electrochemiluminescence (ECL) biosensor for the ultrasensitive detection of -nitrosodimethylamine (NDMA). The biosensor utilizes a triple signal amplification strategy, combining rolling circle amplification (RCA), CRISPR/Cas12a-driven hyperbranched rolling circle amplification (HRCA), and electrostatic repulsion with size exclusion effects from vertically ordered mesoporous silica film (VMSF)/indium tin oxide (ITO) on double-stranded DNA (dsDNA)-Ru(phen) complexes. In this system, aptamers and circular DNA undergo RCA reactions, followed by the CRISPR/Cas12a-mediated HRCA process, producing abundant dsDNA.
View Article and Find Full Text PDFMikrochim Acta
February 2025
Department of Clinical and Military Laboratory Medicine, College of Pharmacy and Laboratory Medicine, Army Medical University), Third Military Medical University, No. 30 Gaotanyan Street, Shapingba District, Chongqing, 400038, China.
The preparation of UiO-66@CdTe@AuNPs composites is presented for the first time, which function as a photoelectrochemical (PEC) sensing matrix and are conjugated to a three-way junction (TWJ). We propose an antibody-based specific recognition-induced neighbor-joining reaction that initiates the assembly of two molecularly designed nucleic acid enzymes (MNAzymes), to release an oligonucleotide that hydrolyzes TWJ through the mechanism of a toehold-mediated strand displacement reaction (TSDR). Subsequently, a hybridization chain reaction application (HRCA)-based dendrimer is formed, which immobilizes a large number of quantum dots to generate a burst effect that reduces the photocurrent signal.
View Article and Find Full Text PDFmSystems
February 2025
Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, China.
This study aimed to elucidate the complement protein C3-mediated host-pathogen interaction in the brain abscess caused by infection. Dual RNA-seq was employed to analyze the transcriptomic differences between C3 deficiency and wild-type mice of induced brain abscess model, and then we investigated the potential regulatory pathways of host interaction mediated by C3 and genes associated with the pathogenesis of brain abscess. Finally, C3 deficient-mice and mutants of were used to verify the specific pathogen-host interaction.
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