Single-Wavelength Laminar Optical Tomography for In Vivo Microvascular Three-Dimensional Imaging.
Annu Int Conf IEEE Eng Med Biol Soc
Published: July 2024
Laminar Optical Tomography (LOT) is a promising non-invasive technique for three-dimensional imaging of complex biological structures, combining high resolution and deep penetration. In this study, a LOT system was assembled using a 520nm continuous-wave laser, exploiting the contrast mechanism provided by the unique optical absorption coefficient of hemoglobin at this wavelength for microvascular imaging. The system utilizes raster scanning to illuminate the target tissue's surface, capturing backscattered light intensity with a multi-channel Photomultiplier Tubes. The inverse problem of light propagation within the tissue is solved to reconstruct a three-dimensional image of microvasculature. Phantom and in-vivo rat ear results demonstrate the system's ability to image micro vessels with diameters of several hundred micrometers within the tissue. The implemented LOT system, known for its simplicity and cost-effectiveness, showcases its potential for applications in microvascular physiological and pathological research, as well as clinical diagnostics.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1109/EMBC53108.2024.10781790 | DOI Listing |
Similar Publications
Safety of intravitreal injections with the mobile laminar airflow device Operio after more than 10.000 injections.
Int Ophthalmol
March 2025
Department of Medicine Surgical, Section of Ophthalmology, University of Perugia, S. Maria Della Misericordia Hospital, 06156, Perugia, Italy.
Purpose: To evaluate the occurrence of complications following intravitreal injections (IVIs) performed in an outpatient clean room (OCR) equipped with the mobile laminar airflow device (LAF) Operio.
Methods: This retrospective study analyzed the data of 10,016 IVIs performed in an OCR from April 2023 to November 2024. All information of each patient was registered, such as diagnosis, drug injected and any occurring complication or adverse events such as endophthalmitis, intraocular inflammation, rhegmatogenous retinal detachment (RRD), vitreous hemorrhages and elevated intraocular pressure.
Non-invasive and fully two-dimensional quantitative visualization of transparent flow fields enabled by photonic spin-decoupled metasurfaces.
Light Sci Appl
March 2025
National Laboratory of Solid-State Microstructures and Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing, 210093, China.
Transparent flow field visualization techniques play a critical role in engineering and scientific applications. They provide a clear and intuitive means to understand fluid dynamics and its complex phenomena, such as laminar flow, turbulence, and vortices. However, achieving fully two-dimensional quantitative visualization of transparent flow fields under non-invasive conditions remains a significant challenge.
View Article and Find Full Text PDFSingle-Wavelength Laminar Optical Tomography for In Vivo Microvascular Three-Dimensional Imaging.
Annu Int Conf IEEE Eng Med Biol Soc
July 2024
Laminar Optical Tomography (LOT) is a promising non-invasive technique for three-dimensional imaging of complex biological structures, combining high resolution and deep penetration. In this study, a LOT system was assembled using a 520nm continuous-wave laser, exploiting the contrast mechanism provided by the unique optical absorption coefficient of hemoglobin at this wavelength for microvascular imaging. The system utilizes raster scanning to illuminate the target tissue's surface, capturing backscattered light intensity with a multi-channel Photomultiplier Tubes.
View Article and Find Full Text PDFImmunofluorescent analysis of exosomes using a microchip filled with transparent antibody-conjugated beads for breast cancer liquid biopsy.
Anal Chim Acta
April 2025
Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun, 130012, China. Electronic address:
Background: Exosomes represent optimal biomarkers for liquid biopsy. Current exosome-based methods are still limited by isolation methods, long processing times, complex structure, low accuracy, large sample volume consumption, and the influence of chemical reagents on the analysis results.
Result: To overcome these challenges, a novel microfluidic approach that employs continuous, rapid, and efficient processes is proposed for the capture and enriching exosomes using the optical transparent antibody-conjugated microbeads within the chip.
RPE Basal Lamina Biology and Pathophysiology Related to Age-Related Macular Degeneration.
Adv Exp Med Biol
February 2025
Department of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, MN, USA.
Age-related macular degeneration (AMD) is a progressive eye disease that primarily affects the central part of the retina and is a leading cause of vision impairment worldwide (Wong WL, Su X, Li X, Lancet Glob Health 2:e106-116, 2014). Sub-retinal pigment epithelium (RPE) deposits represent a broadly defined term encompassing compositionally and anatomically distinct protein and lipid aggregates (i.e.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!
© LitMetric 2025. All rights reserved.