Reusable magnetic alginate nanocomposite with immobilized Pseudozyma antarctica yeast cells offers the potential for bioremediation and detoxification of reactive black 5 dye.

Azo dyes in wastewater of textile, paper and pulp, cosmetics, and leather tanning industries have toxicity and mutagenicity for all living organisms. The aim of the current research was the study of free and immobilized yeast cells of Pseudozyma antarctica on the beads of magnetic alginate nanocomposite to decolorize and detoxify Reactive Black 5 (RB5) as a representative of azo dyes. This yeast decolorized a high concentration of RB5 (2500 mg/L) with 82.30 % decolorization efficiency. The beads containing immobilized yeast cells were used for decolorization in consecutive cycles, and the decolorization efficiency of the beads after four cycles was more than 67 %. UV-visible and FTIR spectroscopy studies showed that the azo bond in RB5 was removed and destroyed by yeast activity. The azo bond of RB5 is destroyed in the biodegradation mechanism which confirms the decolorization of RB5 by P. antarctica via the biodegradation mechanism. According to the phytotoxicity results, RB5 decolorization by P. antarctica reduced the toxicity of the dye. This is the first study on the decolorizing and detoxifying potentials of P. antarctica and introduces a promising candidate for the treatment of wastewater containing azo dyes. Furthermore, its successful immobilization on magnetic alginate nanocomposite and its convenient consecutive uses make it more attractive for environmental applications.