In both normal and pathological conditions, excess spermatogenic cells in the testicular tissue are known to be removed by the mechanism of apoptosis. There are a few studies on spermatogenic cell apoptosis in avian species. Therefore, the purpose of this study was to evaluate the rate of spermatogenic cell apoptosis in three different age groups of the local domestic chicken (Gallus gallus domesticus). Samples were collected from the testes of domestic chicken at three different reproductive stages; pre-pubertal, pubertal, and adult. The samples were subjected to transmission electron microscopy (TEM) and flow cytometry evaluations. TEM results revealed the morphological characteristics of apoptotic cells which included irregular nuclear and plasma membranes in the early stages of apoptosis, nuclear membrane rupture, nuclear material condensation, and fragments of apoptotic bodies in the later stages of apoptosis. The flow cytometry results revealed a significant difference between the mean percentage of apoptotic spermatogenic cells for the three age groups (P < 0.05). Post hoc analysis revealed a significant difference in the adult age group relative to the pre-pubertal age group. However, there was no significant difference between apoptotic spermatogenic cells of the pre-pubertal and the pubertal, and between the pubertal and the adult age groups. In conclusion, the present study revealed a gradual increase in the rate of apoptotic spermatogenic cells in the testes of domestic chicken during the pre-pubertal, pubertal, and adult age groups.
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http://dx.doi.org/10.1016/j.psj.2025.104953 | DOI Listing |
Echocardiography
March 2025
Department of Physical Therapy, Faculty of Health Science, Kyorin University, Mitaka City, Tokyo, Japan.
Purpose: Central hypovolemia is considered to lead to a compensatory increase in cardiac contractility. From a physiological perspective, left ventricular (LV) twisting motion, which plays an important role in maintaining cardiac output, should be enhanced during central hypovolemia, but previous studies have shown inconsistent findings. Using 3D echocardiography, we tested the hypothesis that the LV twisting and untwisting motion would be enhanced during severe central hypovolemia.
View Article and Find Full Text PDFJMIR Hum Factors
March 2025
Department of Internal Medicine, Seoul National University Bundang Hospital, Seoul National University College of Medicine, 82, Gumi-ro 173 Beon-gil, Bundang-gu, Gyeonggi-do, Seongnam-si, 13620, Republic of Korea, 82 317877085.
Background: Ward rounds are an essential component of inpatient care. Patient participation in rounds is increasingly encouraged, despite the occasional complicated circumstances, especially in acute care settings.
Objective: This study aimed to evaluate the effect of real-time ward round notifications using SMS text messaging on the satisfaction of inpatients in an acute medical ward.
Clin Transplant
March 2025
Division of Cardiac Surgery, CardioVascular Center, Tufts Medical Center, Boston, Massachusetts, USA.
Background: This study aims to analyze the patient characteristics, clinical outcomes, and contemporary trends concerning type A aortic dissection (TAAD) in previous recipients of abdominal solid organ transplantation (ASOT) in the United States.
Methods: The National Inpatient Sample was queried to identify all patients aged ≥18 with TAAD and a history of ASOT (TAAD-ASOT) between 2002 and 2015Q3 using ICD-9 diagnosis and procedure codes. Baseline characteristics and in-hospital outcomes were compared between TAAD-ASOT patients and TAAD patients without a history of ASOT (TAAD-non-ASOT).
Echocardiography
March 2025
Division of Pediatric Cardiology, Department of Pediatrics, UCLA Mattel Children's Hospital, David Geffen School of Medicine at UCLA, Los Angeles, California, USA.
Objective: To determine the sensitivity, specificity, and false-positive rate among fetuses suspected prenatally to have coarctation of the aorta (CoA) using size and shape measurements of the fetal heart from the four-chamber view (4CV).
Methods: This was a retrospective study of 108 fetuses identified by pediatric cardiologists to be at risk for CoA. 4CV s from the last antenatal ultrasound performed by the cardiologists were analyzed.
JMIR Med Educ
March 2025
Division of Pulmonary, Critical Care, & Sleep Medicine, Department of Medicine, NYU Grossman School of Medicine, 550 First Avenue, 15th Floor, Medical ICU, New York, NY, 10016, United States, 1 2122635800.
Background: Although technology is rapidly advancing in immersive virtual reality (VR) simulation, there is a paucity of literature to guide its implementation into health professions education, and there are no described best practices for the development of this evolving technology.
Objective: We conducted a qualitative study using semistructured interviews with early adopters of immersive VR simulation technology to investigate use and motivations behind using this technology in educational practice, and to identify the educational needs that this technology can address.
Methods: We conducted 16 interviews with VR early adopters.
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