Introduction: Plasma HIV viral load tests have been widely used in clinical practice to monitor treatment success or failure. Inappropriate mixing with anticoagulants and inaccessibility of plasma samples in certain clinical services (such as antenatal care services) might hinder HIV diagnosis and treatment services. Considering that serum has higher stability and availability in prenatal care services, periodic monitoring of serum HIV viral load might be an alternative approach. Thus, this study aimed to evaluate the plasma and serum HIV-1 viral load measurements among HIV-positive individuals in Northwest Ethiopia.
Methods: An institution-based analytical cross-sectional study was conducted on 74 paired plasma and serum samples from May to August 2020 at the HIV Treatment Center, Northwest Ethiopia. Four milliliters of paired venous blood were collected to harvest plasma and serum. HIV-1 RNA was extracted and quantified using the Roche COBAS AmpliPrep/COBAS TaqMan assay. Data were analyzed using SPSS version 20. Paired sample t-tests and Pearson's correlation were employed to observe the mean differences and associations between sample measurements, respectively. Bland-Altman and linear regression models were computed to demonstrate the level of agreement and proportional bias, respectively. A p-value of ≤ 0.05 with a 95% confidence interval was considered statistically significant.
Results: From a total of 74 HIV-positive individuals, 49 (66.2%) were females. The mean ± SD age was 36.3 (10.1) years. The mean difference ± SD of plasma and serum HIV-1 viral load was 0.07 ± 0.75 log copies/mL, (p = 0.428). A strong association with a significant linear correlation (r = 0.862) (p < 0.001) and a high level of agreement were observed between the sample measurements using Pearson's correlation and a Bland-Altman plot, respectively.
Conclusions: The current study highlights an alternative application of serum-based HIV viral load quantification to enhance the rate of HIV diagnosis and treatment monitoring coverage.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875351 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0315717 | PLOS |
Publication Analysis
Top Keywords
Similar Publications
Longitudinal CNS and systemic T-lymphocyte and monocyte activation before and after antiretroviral therapy beginning in primary HIV infection.
Front Immunol
March 2025
Department of Neurology, Yale University School of Medicine, New Haven, CT, United States.
Background: Trafficking of immune cells to the central nervous system is hypothesized to facilitate HIV entry and immune-induced neuronal injury and is mediated by surface proteins such as chemokine receptors and α4 integrin. We longitudinally assessed immune cell activation and surface marker expression in cerebrospinal fluid (CSF) and blood and their relationship with CSF HIV RNA beginning during primary HIV infection (PHI) before and after antiretroviral therapy (ART).
Methods: Longitudinal paired blood and CSF were obtained in ART-naïve PHI (<12 month since infection) participants; some independently initiated ART during follow up.
HIV controllers: hope for a functional cure.
Front Immunol
March 2025
Beijing Key Laboratory for HIV/AIDS Research, Clinical and Research Center for Infectious Diseases, Beijing Youan Hospital, Capital Medical University, Beijing, China.
Elite controllers (ECs) and post-treatment controllers (PTCs) represent important models for achieving a functional cure for HIV. This review synthesizes findings from immunological, genetic, and virological studies to compare the mechanisms underlying HIV suppression in ECs and PTCs. Although ECs maintain viral control without antiretroviral therapy (ART), PTCs achieve suppression following ART discontinuation.
View Article and Find Full Text PDFDose determination of VV116 in COVID-19 patients with severe liver dysfunction: a case report.
Front Med (Lausanne)
February 2025
Department of Pharmacy, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
VV116 is an oral antiviral drug against SARS-CoV-2, known for its favorable efficacy and safety profile. But its application in patients with severe liver dysfunction has not been evaluated. Here, we report a case in which a patient with aplastic anemia and liver impairment (recovery phase of acute liver failure) was infected with SARS-CoV-2.
View Article and Find Full Text PDFHigh prevalence of low-level viremia among infants initiated on antiretroviral drugs following mother-to-child transmission of HIV.
AIDS Res Ther
March 2025
Joint Clinical Research Center, Plot 101 Lubowa Hill, Kampala, Uganda.
Background: With the current elimination of mother to child transmission (EMTCT) of HIV, the number of HIV-positive newborns has greatly reduced. Some countries have successfully eliminated HIV infections among newborn babies.
Methods: This study was nested within the DRIBS (Drug Resistance testing among Infants at Baseline Study), which enrolled 100 infants at the time of treatment initiation between 2017 and 2023.
Altered KEGG pathways are associated with different immunologic responses to antiretroviral therapy in HIV-infected men who have sex with men.
BMC Infect Dis
March 2025
School of Public Health, Fudan University, Shanghai, China.
Background: The association between Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways and immunologic non-response among people living with HIV (PLHIV) on antiretroviral therapy (ART) is not well documented. This study aimed to characterize KEGG metabolic pathways among HIV-infected men who have sex with men (MSM) with different immunologic responses.
Methods: We recruited HIV-uninfected MSM (healthy controls, HC) and HIV-infected MSM on ART > 24 months in Guangzhou, June-October 2021.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!