Background: Esophageal Squamous Cell Carcinoma (ESCC) presents a serious global health challenge, ranking among the most prevalent cancers worldwide. Small mothers against decapentaplegic 2 (SMAD2) and SMAD4 play a significant role in various types of cancer.
Methods: This study performed relative mRNA expression level profiling of SMAD2 and SMAD4 using Real time quantitative polymerase chain reaction (qPCR) in tissue and blood of ESCC patients and analyzed their associations with numerous clinical and lifestyle parameters for evaluating prognostic significance along with survival and hazard outcomes.
Results: SMAD2 and SMAD4 relative expression level showed downregulation in both tissue (85% and 87% respectively) and blood samples (80% and 79% respectively), and a significant positive correlation (p < 0.05) between their relative expression level was observed in both tissue and blood levels. Various clinicopathological parameters and food habits revealed significant association (p < 0.05) with SMAD2 and SMAD4 relative expression level. While analyzing survival and hazard in ESCC patients, various parameters revealed significant association (p < 0.05) in univariate model and histopathology grade, node stage, stage of metastasis, betel nut consumption, smoked food consumption and altered SMAD2 and SMAD4 relative expression level in tissue samples revealed significant association (p < 0.05) in the multivariate model, indicating their direct association with ESCC patients' survival and this makes them reliable predictors for ESCC prognosis.
Conclusions: This study's results revealed that downregulation of SMAD2 and SMAD4 is associated with poor prognosis and ESCC progression emphasizing their potential as potent prognostic factors for survival prediction as well as reliable biomarkers for screening.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s11033-025-10390-w | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A positive feedback loop between SMAD3 and PINK1 in regulation of mitophagy.
Cell Discov
March 2025
Faculty of Healthy Sciences, Ministry of Education Frontiers Science Center for Precision Oncology, University of Macau, Macau, China.
PTEN-induced kinase-1 (PINK1) is a crucial player in selective clearance of damaged mitochondria via the autophagy-lysosome pathway, a process termed mitophagy. Previous studies on PINK1 mainly focused on its post-translational modifications, while the transcriptional regulation of PINK1 is much less understood. Herein, we reported a novel mechanism in control of PINK1 transcription by SMAD Family Member 3 (SMAD3), an essential component of the transforming growth factor beta (TGFβ)-SMAD signaling pathway.
View Article and Find Full Text PDFMolecular Mechanisms of Neutrophil Extracellular Traps in Promoting Gastric Cancer Epithelial-Mesenchymal Transition Through SERPINE-1 Expression.
J Biochem Mol Toxicol
March 2025
Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin City, China.
Gastric cancer remains a significant global health concern, with its progression and metastasis often associated with epithelial-mesenchymal transition (EMT). This study investigated the role of neutrophil extracellular traps (NETs) in promoting gastric cancer EMT by regulating SERPINE-1 expression, which encodes plasminogen activator inhibitor-1 (PAI-1). Western blot and immunohistochemistry were used to detect protein expression.
View Article and Find Full Text PDFDownregulation of SMAD2 and SMAD4 is associated with poor prognosis and shorter survival in esophageal squamous cell carcinoma.
Mol Biol Rep
March 2025
Department of Bioengineering and Technology, Gauhati University, Guwahati, Assam, 781014, India.
Background: Esophageal Squamous Cell Carcinoma (ESCC) presents a serious global health challenge, ranking among the most prevalent cancers worldwide. Small mothers against decapentaplegic 2 (SMAD2) and SMAD4 play a significant role in various types of cancer.
Methods: This study performed relative mRNA expression level profiling of SMAD2 and SMAD4 using Real time quantitative polymerase chain reaction (qPCR) in tissue and blood of ESCC patients and analyzed their associations with numerous clinical and lifestyle parameters for evaluating prognostic significance along with survival and hazard outcomes.
Exploring anticancer potential of betanin in DMBA-induced oral squamous cell carcinoma: an in silico and experimental study.
Naunyn Schmiedebergs Arch Pharmacol
February 2025
Central Animal House Government Medical College and Hospital Cuddalore, Chidambaram, Annamalainagar, 608002, Tamil Nadu, India.
In addition to being able to fight cancer, betanin (BTN) has amazing natural antioxidant and peroxy-radical scavenging properties. 7,12-Dimethylbenz[a]anthracene (DMBA) can impair the activities of enzymes accountable for breaking down xenobiotics and can also cause lipid peroxidation. The study's goal was to find out if betanin could protect against these problems.
View Article and Find Full Text PDFPhosphorylation of FOXN3 by NEK6 promotes pulmonary fibrosis through Smad signaling.
Nat Commun
February 2025
Anhui Province Key Laboratory of Respiratory Tumor and Infectious Disease, Department of Respiratory and Critical Care Medicine, First Affiliated Hospital, Bengbu Medical University, Bengbu, China.
The transcriptional repressor FOXN3 plays a key role in regulating pulmonary inflammatory responses, which are crucial in the development of pulmonary fibrosis. However, its specific regulatory function in lung fibrosis remains unclear. Here, we show that FOXN3 suppresses pulmonary fibrosis by inhibiting Smad transcriptional activity.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!