Viscosity is an important physiological parameter closely associated with various cellular processes and diseases. Several fluorescence probes responsive to viscosity have been developed, demonstrating high sensitivity specifically towards tumor tissues. However, the underlying core mechanism of this highly potential responsive signal has been a subject of debate, as highly sensitive probes encounter excessive environmental interferences in complex tumor tissues. Therefore, we have developed a viscosity-responsive fluorescence probe based on the classical TICT mechanism (twisted intramolecular charge transfer) as a research tool. This probe features an ultra-wide emission range of 700-1200 nm in the near-infrared spectrum, strong photostability, and simultaneous targeting of mitochondria and lysosomes. Through in-depth analysis, we have revealed the intrinsic mechanisms underlying its functionality, demonstrating that the major contributor to the fluorescence change of responsive probes during imaging is the inherent state of cells rather than the tumor microenvironment or the cell type. Our findings provide a theoretical foundation for the continued exploration and application of viscosity-responsive probes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/d4tb02067g | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cryptophycin unit B analogues.
Beilstein J Org Chem
March 2025
Department of Chemistry, Organic and Bioorganic Chemistry, Bielefeld University, Universitätsstraße 25, 33615 Bielefeld, Germany.
Drug conjugates using toxic payloads are a promising approach for selectively combating cancer while sparing healthy tissue. The lack of highly cytotoxic and at the same time selective therapeutics against cancer is an ongoing challenge. Cryptophycins are a class of cyclic depsipeptides renowned for their high cytotoxicity in the picomolar range often combined with efficacy against multidrug-resistant tumour cell lines.
View Article and Find Full Text PDFDevelopment of a tertiary lymphoid structure-based prognostic model for breast cancer: integrating single-cell sequencing and machine learning to enhance patient outcomes.
Front Immunol
March 2025
Research Laboratory Center, Guizhou Provincial People's Hospital, Guiyang, Guizhou, China.
Background: Breast cancer, a highly prevalent global cancer, poses significant challenges, especially in advanced stages. Prognostic models are crucial to enhance patient outcomes. Tertiary lymphoid structures (TLS) within the tumor microenvironment have been associated with better prognostic outcomes.
View Article and Find Full Text PDFSingle-cell RNA-sequencing reveals cellular heterogeneity and immune microenvironment characteristics between ocular adnexal mucosa-associated lymphoid lymphoma and IgG4-related ophthalmic disease.
Front Immunol
March 2025
Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Introduction: The molecular pathogenesis of ocular adnexal mucosa-associated lymphoid tissue (MALT) lymphoma and IgG4-related ophthalmic disease (IgG4-ROD) remains incompletely understood. Differentiating between the two diseases is vital given that the diagnostic evaluation and treatment approaches can vary significantly; this difficulty in distinction is exacerbated by the absence of specific biomarkers. This study aimed to investigate the differences between these two diseases based on their cellular composition, transcriptional heterogeneity, and the immune microenvironment using single-cell RNA transcriptional sequencing (scRNA-seq) technology.
View Article and Find Full Text PDFPGAM5 Modulates Macrophage Polarization, Aggravating Inflammation in COPD via the NF-κB Pathway.
Int J Chron Obstruct Pulmon Dis
March 2025
Department of Respiratory Medicine, The second Affiliated Hospital, Hainan Medical University, Haikou, 570100, People's Republic of China.
Background: Chronic obstructive pulmonary disease (COPD) has emerged as a very consequential issue threatening human life and health; therefore, research on its pathogenesis is urgently needed. A prior investigation discovered a significant elevation in the phosphoglycerate mutase 5 (PGAM5) expression in the lung tissue of COPD smoking patients. This rise in expression is closely associated with COPD severity.
View Article and Find Full Text PDFPostmastectomy Late Breast Reconstruction with Transverse Rectus Abdominis Flap after Primary Closure with Latissimus Dorsi.
Indian J Plast Surg
February 2025
Breast Surgery Department, Hospital de Câncer de Muriaé, Muriaé, MG, Brasil.
Locally advanced breast cancer (LABC) is common in countries where organized screening is not effective. Although neoadjuvant therapy increases resectability, many patients undergo mastectomy and, in some cases, flaps are necessary for primary closure of the chest wall. Despite a worse prognosis, some of these women will achieve long-term survival and may require breast reconstruction.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!