C-C chemokine receptor type 7 (CCR7) is a member of the G protein-coupled receptor family and functions as a lymph node-homing receptor for immune cells. Upon ligand binding, CCR7 promotes the migration of immune cells to secondary lymphoid organs. In cancers, CCR7 has been revealed as a critical molecule in lymph node metastasis. Consequently, anti-CCR7 monoclonal antibodies (mAbs) have been developed as cancer therapeutic agents. In this study, we established an anti-mouse CCR7 (mCCR7) mAb, CMab-7 (rat IgG, kappa) using the Cell-Based Immunization and Screening (CBIS) method. CMab-7 demonstrated high sensitivity in flow cytometry. The dissociation constant ( ) value of CMab-7 was determined to be 2.5 × 10⁻⁹ M for mCCR7-overexpressed Chinese hamster ovary-K1 (CHO/mCCR7) cells. Furthermore, CMab-7 detected mCCR7 with high sensitivity in western blot and immunohistochemistry. CMab-7, developed by the CBIS method, accelerates the development of CCR7-targeted antibody therapies and cancer diagnostics.
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| http://dx.doi.org/10.1016/j.bbrep.2025.101948 | DOI Listing |
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