This comprehensive evaluation evaluates the activity of total flavonoids from Atractylodes macrocephala (AMF) in treating acute lung injury (ALI) and explores the mechanism of action of AMF total flavonoids in treating ALI. Method: UV spectrophotometry was used to determine the in vitro antioxidant activity of total flavonoids in AMF; Establishment of ALI model mice induced by lipopolysaccharide (LPS); Analysis of total flavonoid composition and blood migration components in AMF based on UHPLC-MS/MS technology; Analyze the active compounds, targets, and mechanisms of action of AMF total flavonoids in treating ALI through serum drug chemistry combined with network pharmacology. The results of in vitro antioxidant combined with in vivo anti-inflammatory experiments showed that AMF total flavonoids exhibited excellent therapeutic effects on ALI. Through network pharmacology, it was found that AMF treatment for ALI mainly targets quercetin, kaempferol, and 5-hydroxy-3,3 ', 4', 6,7,8-hexamethoxyflavone through AKT1, IL6, and TNF, and regulates inflammatory response through the PI3K/AKT signaling pathway. Conclusion: This experiment reveals the mechanism of action of total flavonoids in AMF fruits in treating ALI, providing a new pathway for utilizing AMF as a new food resource and offering new ideas for the research of new drugs for the prevention and treatment of ALI.
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