Glyphosate is the most used herbicide globally, but little is known of its prevalence in the Australian environment or its behaviour during wastewater treatment. This study examined the concentration of glyphosate and its primary biodegradation product, aminomethylphosphonic acid (AMPA), in influent and effluent from 22 Australian wastewater treatment plants (WWTPs) to inform exposure risks. Glyphosate was detected in all 22 WWTP influent samples, with concentrations ranging from 0.37 to 370 μg/L (mean: 22 ± 76 μg/L). In treated effluent, glyphosate was found in 82 % of samples with concentrations ranging from
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