Background: Rheumatoid arthritis (RA) is categorized as an autoimmune condition. Bone marrow-derived mesenchymal stromal cell (BMSC) derived exosome (BMSC-Exo) exert vital character in RA. We aimed to investigate the regulatory mechanism of BMSC-Exo in alleviating RA.
Methods: BMSC was isolated from mouse bone marrow. Collagen-induced arthritis (CIA) was induced by injecting bovine type II collagen and complete Freund's adjuvant. Arthritis score, incidence, and withdrawal threshold were assessed. Hematoxylin-eosin staining was used to observe knee joint damage. CD4 T cells were isolated from the spleen, and T helper 17 (Th17) proportions were measured by flow cytometry. Caspase-1 activity was assessed.
Results: BMSC-Exo injection reduced arthritis score and incidence of arthritis, and elevated the withdrawal threshold of CIA mice. BMSC-Exo also alleviated knee damage in CIA mice and reduced the Th17 proportion. BMSC-Exo down-regulated inflammatory cytokine levels, as well as caspase-1 activity. BMSC-Exo up-regulated PR Domain Zinc Finger Protein 1 (PRDM1) levels. PRDM1 knockdown in BMSC down-regulated PRDM1 expression in Exo but did not affect up-regulated PRDM1 expression in CD4 T cells. In vivo, BMSC-Exo affected RA pathology by acting on PRDM1.
Conclusions: BMSC-Exo improved RA by promoting PRDM1 expression in CD4 T cells and inhibiting Th17 cell differentiation.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11881839 | PMC |
| http://dx.doi.org/10.1080/17460751.2025.2469426 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fc-mediated immune stimulating, pro-inflammatory and antitumor effects of anti-HER2 IgE against HER2-expressing and trastuzumab-resistant tumors.
J Immunother Cancer
March 2025
St. John's Institute of Dermatology, School of Basic & Medical Biosciences & KHP Centre for Translational Medicine, King's College London, London, UK
Background: Anti-human epidermal growth factor receptor 2 (HER2) IgG1-based antibody therapies significantly improve cancer prognosis, yet intrinsic or acquired resistance to fragment antigen-binding (Fab)-mediated direct effects commonly occurs. Most resistant tumors retain antigen expression and therefore remain potentially targetable with anti-HER2 therapies that promote immune-mediated responses. Tumor-antigen-specific IgE class antibodies can mediate powerful immune cell-mediated effects against different cancers and have been shown to activate IgE Fc receptor-expressing monocytes.
View Article and Find Full Text PDFGPR171 restrains intestinal inflammation by suppressing FABP5-mediated Th17 cell differentiation and lipid metabolism.
Gut
March 2025
Department of Gastroenterology, Shanghai Tenth People's Hospital, Shanghai, China
Background: GPR171 suppresses T cell immune responses involved in antitumour immunity, while its role in inflammatory bowel disease (IBD) pathogenesis remains unclear.
Objective: We aimed to investigate the role of GPR171 in modulating CD4 T cell effector functions in IBD and evaluate its therapeutic potential.
Design: We analysed GPR171 expression in colon biopsies and peripheral blood samples from patients with IBD and assessed the impact of GPR171 on CD4 T cell differentiation through administration of its endogenous ligand (BigLEN).
Background: The pathogenesis of female pelvic floor polypropylene mesh complications is unclear as trials evaluating explanted mesh have not included asymptomatic controls.
Objectives: To compare explanted polypropylene mesh from those with and without mesh complications to determine the pathogenesis of the complications.
Methods: Between August 2019 and July 2020 66 participants attending Wesley and Royal Brisbane and Women's Hospital Urogynecology department with mesh complications and 15 undergoing repeat prolapse and/or continence surgery after prior polypropylene mesh implantation were included.
CD4 T cells take center stage during dengue infection.
Cell Rep
March 2025
Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montréal, QC, Canada; Département de médicine, Université de Montréal, Montréal, QC, Canada. Electronic address:
Ansari et al. identified a subset of CD4 T cells (CXCR5PD-1) resembling T follicular helper (Tfh) cells in patients with severe dengue. This subset helps B cell responses via IL-21 and can also differentiate into cytotoxic CD4 T cells.
View Article and Find Full Text PDFDistinct features of a peripheral T helper subset that drives the B cell response in dengue virus infection.
Cell Rep
March 2025
Vaccine Immunology Laboratory, National Institute of Immunology, New Delhi 110067, India. Electronic address:
Dengue-virus-induced humoral immunity can increase the risk of severe disease, but the factors influencing this response are poorly understood. Here, we investigate the contribution of CD4 T cells to B cell responses in human dengue infection. We identify a dominant peripheral PD-1 T cell subset that accumulates in severe patients and could induce B cell differentiation via interleukin-21 (IL-21)-related pathway.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!