The transition to a more plant-based diet embraces a higher consumption of diversified pulses. Understanding the chemical composition of pulses is crucial to decipher their biofunctionality. This study analyzed 14 different cultivars of 4 types of pulses (pea, lentil, faba bean, and lupin) using NMR-based metabolomics. Sucrose, glutamate, and citrate were the metabolites representing the most abundant polar chemical classes (carbohydrates, amino acids, and organic acids). Lupin had a higher content of carbohydrates, and a lower content of free amino acids than the other species. Differences among the cultivars related to carbohydrates were found for peas and lentils, which was reflected in variations in their metabolic pathway potential. Faba beans showed highest concentrations of phenolic compounds. Correlation with data from descriptive sensory profiling enabled pinpointing several amino acids and some organic acids that contributed to explain variations in perceived smell and taste among the cultivars.
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