A novel approach for estimating postmortem intervals under varying temperature conditions using pathology images and artificial intelligence models.

Estimating the postmortem interval (PMI) is a critical yet complex task in forensic investigations, with accurate and timely determination playing a key role in case resolution and legal outcomes. Traditional methods often suffer from environmental variability and subjective biases, emphasizing the need for more reliable and objective approaches. In this study, we present a novel predictive model for PMI estimation, introduced here for the first time, that leverages pathological tissue images and artificial intelligence (AI). The model is designed to perform under three temperature conditions: 25 °C, 37 °C, and 4 °C. Using a ResNet50 neural network, patch-level images were analyzed to extract deep learning-derived features, which were integrated with machine learning algorithms for whole slide image (WSI) classification. The model achieved strong performance, with micro and macro AUC values of at least 0.949 at the patch-level and 0.800 at the WSI-level in both training and testing sets. In external validation, micro and macro AUC values at the patch-level exceeded 0.960. These results highlight the potential of AI to improve the accuracy and efficiency of PMI estimation. As AI technology continues to advance, this approach holds promise for enhancing forensic investigations and supporting more precise case resolutions.