An electrochemical biosensor for the detection of BRCA1 based on MOF-derived CeO@CuS nanosheets with high electrocatalytic HO reduction performance.

Background: Since the incidence of breast cancer ranks first among female malignant tumors, early diagnosis and treatment of breast cancer are crucial to women's health. Therefore, the detection and monitoring of breast cancer susceptibility gene (BRCA1) is particularly important. In this paper, an electrochemical biosensor was developed to detect BRCA1 based on MOF-derived CeO@CuS nanosheets with efficient electrocatalytic activity of HO reduction.

Results: CeO nanotube derived from MOF possessed excellent charge transfer ability and high specific surface area, making it an ideal substrate for loading CuS nanosheets. The combination of CeO and CuS enhanced the electrocatalytic activity and conductivity of HO redox reaction. Then gold nanoparticles (Au) were introduced to synthesize Au@CeO@CuS to further improve conductivity and electrocatalytic activity. Based on the excellent properties of the above synthetic materials, the non-enzymatic electrochemical sensing platform for detecting BRCA1 were constructed. The proposed method demonstrated wide linearity ranges and low detection limits for detecting BRCA1 (0.1 pmol/L - 10 nmol/L, 0.02 fmol/L), respectively.

Significance: These showed that the sensing strategy provides a new approach for the detection of BRCA1 and has important significance for the early diagnosis and treatment of breast cancer.