Microplastic contamination in threatened wild felids of India: Understanding environmental uptake, feeding implications, and associated risks.

While the presence of microplastics (MPs, <5 mm) in various aquatic organisms is well-documented, studies on the accumulation of MPs in terrestrial predators remain limited worldwide, including in India. This study aims to evaluate, for the first time, the occurrence of MPs in the scat of mid-sized felids-fishing cat and jungle cat-from their overlapping habitat in the Gangetic Estuary of India. The risk assessment of MPs and management recommendation for MP mitigation was also discussed in this context. Notably, our study is the first to report the presence of MPs and mesoplastics in fishing cat from India and jungle cat globally. The abundance of MPs was found to be higher in jungle cat (12.6 ± 1.93 MP/g d.w) compared to fishing cat (10.5 ± 2.12 MP/g d.w) in the Gangetic estuary. Furthermore, fiber-shaped (70.37%) and 1-5 mm-sized (47.73%) MPs predominated in both felid species, while fiber bundles were observed only in jungle cat. Red-colored MPs (27.62%) were predominantly found in fishing cat, whereas transparent MPs (33.33%) were more common in jungle cat. Scanning electron microscopy revealed possible environmental and digestive degradation marks on the MPs. A total of seven synthetic and one natural polymer were identified, with Ethylene Vinyl Alcohol (55.56%) being predominant in fishing cat and Polyethylene (33.33%) more common in jungle cat. Polymer risk assessment indicated that the MPs in fishing cat fall into the danger category, Group IV (PHI 100-1000), while jungle cat possess high threat under extreme danger category, Group V (PHI >1000). The observed MPs and mesoplastics in felids probably come from adjacent environmental uptake and/or accumulate through trophic transfer from prey items. The evidence of MPs in felids may pose a threat to the big cat-Royal Bengal tigers in the Sundarbans. Therefore, various landscape-based policy implementations are recommended to mitigate MP pollution.