Objective: The aim of the present study was to characterize the antimicrobial and anti-inflammatory activities of postbiotics from lactic acid bacteria against .
Material And Methods: The anti- activity of postbiotics from the CERELA culture collection was assessed by measuring changes in the expression of key host proteins by ELISA and qPCR, the proteolytic activity by a fluorescence and a spectrophotometric method and virulence factors from by qPCR.
Results: Even though CRL1522 and CRL1363 exhibit only a discrete antibacterial activity against , the cell-free supernatants of these strains significantly reduced -induced secretion of interleukins IL-6 and IL-8 by keratinocytes and TNF-α and IL-6 by U937 macrophage-like cells. More importantly, arginine-gingipain (Rgp) protease activity was markedly reduced by both lactic acid bacteria (LAB) strains. This finding is particularly interesting because it means that both LAB might prevent the ulterior citrullination of peptides and the consequent generation of autoantibodies. The expression of and was also significantly downregulated in macrophages.
Conclusion: Postbiotics from CRL1522 and CRL1363 rise as suitable candidates for antagonizing the periodontopathogen , since they were able to reduce the expression of proinflammatory cytokines and the protein degradation induced by this pathogen. We propose that postbiotics from these LAB could potentially halt the progression of periodontitis based on this study.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11864006 | PMC |
| http://dx.doi.org/10.1080/20002297.2025.2469894 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Janus Membrane-Based Wearable Dual-Channel SERS Sensor for Sweat Collection and Monitoring of Lactic Acid and pH Levels.
Anal Chem
March 2025
School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, 44 West Culture Road, Ji'nan 250012, China.
Sweat, as a metabolic byproduct, encompasses a diverse array of molecular information pertinent to our physiological states and overall health. The extraction of minute quantities of sweat, coupled with sensitive monitoring and identification of its internal molecular components, constitutes an effective strategy for assessing bodily conditions. We engineer a Janus membrane utilizing electrospinning techniques for application on human skin to facilitate sweat collection.
View Article and Find Full Text PDFHighly Adhesive ZJ316: Structural Insights of Lipoteichoic Acid and Its Anti-Inflammatory Properties.
J Agric Food Chem
March 2025
Key Laboratory for Food Microbial Technology of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang 310018, People's Republic of China.
Lactic acid bacteria are widely recognized for their probiotic properties, and their adhesion to the gastrointestinal tract is a prerequisite for their probiotic functions. This investigation aimed to screen a highly adherent strain and explore the impact of its surface lipoteichoic acid (LTA) on strain adhesion to intestinal epithelial cells and the immunomodulatory activity. Results demonstrated that ZJ316 exhibited remarkable surface properties and superior adhesion to enterocytes, and the fluorescent labeling revealed that ZJ316 predominantly adhered to the cecum in mice.
View Article and Find Full Text PDFHOTAIR Participation in Glycolysis and Glutaminolysis Through Lactate and Glutamate Production in Colorectal Cancer.
Cells
March 2025
Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Tlalnepantla 54090, Mexico.
Metabolic reprogramming plays a crucial role in cancer biology and the mechanisms underlying its regulation represent a promising study area. In this regard, the discovery of non-coding RNAs opened a new regulatory landscape, which is in the early stages of investigation. Using a differential expression model of HOTAIR, we evaluated the expression level of metabolic enzymes, as well as the metabolites produced by glycolysis and glutaminolysis.
View Article and Find Full Text PDFSkim Milk Culture of SBT0309 Increases Intestinal Alkaline Phosphatase Activity and Inhibits Lipopolysaccharide-Induced Interleukin-8 Production in Intestinal Epithelial Cells.
Cells
February 2025
Milk Science Research Institute, MEGMILK SNOW BRAND Co., Ltd., 1-1-2 Minamidai, Kawagoe-shi, Saitama 350-1165, Japan.
Background/objectives: Intestinal alkaline phosphatase (IAP) is an enzyme expressed in the intestinal brush border, which may exert anti-inflammatory effects by detoxifying lipopolysaccharides (LPSs), thereby preventing metabolic disorders. Various food components have been reported to influence IAP activity. However, few studies have evaluated the effects of fermented milk on IAP activity.
View Article and Find Full Text PDFA Biomimetic Sweeping Microrobot for Active Therapy of Ulcerative Colitis.
Adv Mater
March 2025
NMPA Key Laboratory for Research and Evaluation of Drug Metabolism & Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China.
Overproduction of pathogenic cell-free DNA (cfDNA) and reactive oxygen species (ROS) plays crucial roles in the onset and perpetuation of ulcerative colitis (UC). Inspired by sweeping robots, a magnesium@polylactic acid-glycolic acid copolymer@polyethylenimine (Mg@PLGA@PEI) microswimmer capable of cleaning off deleterious disease triggers along its path of progress is designed. Mg@PLGA@PEI is successfully synthesized by adopting a core-shell structure with a small opening which allows for Mg-water reaction.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!