sp. nov., isolated from the rhizosphere of rice and genome mining for the prediction of biosynthetic gene clusters.

A Gram-stain-negative, aerobic, rod-shaped, motile and flagellated novel bacterial strain, designated MAHUQ-64, was isolated from the rhizosphere of rice. The colonies were observed to be creamy white-coloured, smooth, spherical and 0.5-1.1 mm in diameter when grown on Reasoner's 2A agar medium for 2 days. Strain MAHUQ-64 was able to grow at 10-40 °C, at pH 5.0-9.5 and in the presence of 0-2.0% NaCl (w/v). The strain was positive for both catalase and oxidase tests. The strain was positive for hydrolysis of l-tyrosine. According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus and is closely related to L3B39 (98.6% sequence similarity) and TTM-24 (98.2%). The novel strain MAHUQ-64 has a draft genome size of 3 827 146 bp (22 contigs), annotated with 3612 protein-coding genes, 74 tRNA and 4 rRNA genes. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-64 and its closest member L3B39 were 86.5 and 33.4%, respectively. genome mining revealed several biosynthetic gene clusters in the genome of the novel strain MAHUQ-64. The genomic DNA G+C content was determined to be 63.4 mol%. The predominant isoprenoid quinone was ubiquinone-8. The major fatty acids were identified as summed feature 3 (comprising C 7 and/or C 6) and C. Based on dDDH, ANI value, genotypic analysis and chemotaxonomic and physiological data, strain MAHUQ-64 represents a novel species within the genus , for which the name sp. nov. is proposed, with MAHUQ-64 (=KACC 22245=CGMCC 1.19000) as the type strain.