Three novel strains, designated LB3P45, LS2P90 and ZS1P70, were isolated from glaciers located on the Tibetan Plateau, PR China. These strains were Gram-stain-negative, aerobic, rod-shaped and yellow or orange coloured. Phylogenetic analysis based on the 16S rRNA gene and genomic sequences indicated that they belong to the genus . The 16S rRNA gene sequence similarity among the three strains ranged from 97.4 to 98.6%. Strain LB3P45 showed 98.9% and 98.7% similarity to CGMCC 1.9230 and JCM 11314, respectively. Strain LS2P90 displayed 99.0% and 98.7% similarity to JCM 11314 and CGMCC 1.9230. Strain ZS1P70 had the highest sequence similarity with JCM 11314 (98.0%) and CGMCC 1.9230 (97.8%). The average nucleotide identity and digital DNA-DNA hybridization values between these strains and their closest relatives were lower than 93.0% and 47.9%, respectively. All three strains contained summed feature 3 (comprising C c and/or C c) as the major fatty acids. Based on phenotypic characteristics, phylogenetic analysis and genotypic data, the three novel species are proposed: sp. nov. (LB3P45=CGMCC 1.11439=NBRC 114819), sp. nov. (LS2P90=CGMCC 1.11685=NBRC 114823) and sp. nov. (ZS1P70=CGMCC 1.24124=NBRC 114829).
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865497 | PMC |
http://dx.doi.org/10.1099/ijsem.0.006694 | DOI Listing |
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