Triacylglycerol Composition of Seed Oil from Berries.

The seeds of are considered a by-product in fruit processing. This study aimed to determine the oil contents in seeds and characterize their triacylglycerol contents through a comparative analysis using three extraction solvent systems: hexane (Soxhlet method), hexane-isopropanol (Hara-Radin method), and methanol-chloroform-water (Bligh-Dyer method). The extracts were analyzed by gas chromatography/mass spectrometry and HPLC. The composition of fatty acids and triacylglycerols was determined, as were the allocation of fatty acids across the -2 and -1,3 positions, tocopherol and tocotrienol profile, and melting behavior through differential scanning calorimetry. Furthermore, the atherogenicity (IA) and thrombogenicity (IT) cardiovascular health indices were also calculated. The oil predominantly contained unsaturated fatty acids, and α-linolenic acid made up 45.8% of the total, along with a reduced n-6/n-3 fatty acid ratio (0.75). The α-linolenoyl chain primarily occupied the -1,3 (45.9%) and -2 (39.1%) positions. γ-tocotrienol was the most abundant tocochromanol. The melting curve of oil suggests the presence of fractions with a low melting point, composed of triacylglycerols containing polyunsaturated fatty acids. The oil exhibits low values for IA and IT of 0.05 and 0.04, respectively. seed oil has potential health benefits thanks to its rich composition in the essential fatty acid, α-linolenic acid, the low proportion of n-6/n-3 fatty acids, and the low values of IA and IT.