The Impact of the Competition on miRNA, Proteins, and Metabolites in the Blood Exosomes of the Horse.

Purpose: Horse racing may cause stress-induced physiological changes and tissue damage in horses, but the changes in miRNA expression, protein expression, and metabolic substances in the plasma exosomes of the horse after racing are still unclear. This study detected miRNA, protein expression, and metabolic substances in the plasma exosomes of horses before and after competition, providing new insights for post-race recovery and care of horses.

Method: Eight three-year-old horses that had undergone training were selected as the research subjects, with four horses that had not competed as the control group and four horses that had participated in the competition for half an hour as the training group. Extract whole blood and separate plasma from two groups of horses, and then extract plasma exosomes; MiRNAs, proteins, and metabolites in extracellular vesicles were detected and analyzed using miRNAomics, proteomics, and metabolomics. P Result: After the competition, the levels of miRNAs related to the cytoplasm and nucleus in horse plasma exosomes increased, and miRNAs related to the transcription and transcriptional regulation of biological processes significantly increased. The levels of proteins related to the cytoplasm and nucleus also increased, and the levels of proteins related to cell signaling function increased, carbohydrates and their metabolites were significantly reduced.

Conclusions: The competition process causes significant changes in the miRNA, proteomics, and metabolomics of plasma exosomes in the horses, which are mainly related to metabolic regulation.