Effects of Black Soldier Fly Larvae Hydrolysate on Culture of Primary Myogenic and Adipogenic Cells Isolated from Broilers for Cultured Meat Development.

Sustainable food resources, including cell-cultured meat and edible insect proteins, are emerging as key solutions to meet future protein demands. This study evaluated the effects of black soldier fly larvae hydrolysate (BLH) on primary cells isolated from broiler leg and breast muscle tissues, as well as abdominal fat tissues. Primary cells isolated from each tissue were characterized for their myogenic and adipogenic (stromal vascular fraction, SVF) properties. Cells were cultured in a basal medium with five percent FBS supplemented with BLH at concentrations ranging from 25 to 300 µg/mL. Leg and breast muscle cells showed significantly enhanced proliferation, as indicated by MTS assay results and cell counts, in the BLH100 group compared to the FBS5 and control groups ( < 0.05). Furthermore, the expression of myogenic markers, including PAX7, NCAM1, MYF5, and MYOD1, was upregulated in leg muscle cells treated with BLH ( < 0.05). For SVFs, BLH50 promoted cell proliferation; however, differentiation decreased as BLH concentration increased. These findings suggest that BLH can enhance the proliferation of primary broiler cells, highlighting its potential applicability in the edible insect and cultured meat industries.