This study presents the first comprehensive proteomic profile of an Italian strain of , a highly heterogeneous white-rot fungal species with significant potential for industrial, nutraceutical, cosmeceutical, and clinical applications. Three protein extraction methods and their impact on yield and resulting protein composition have been compared. Results revealed that the combination of Tris-Cl and urea increases the total protein yield and the variety of enzymatic species related to pivotal pathways. Notably, over 2000 proteins were identified, including enzymes involved in the growth and development of mycelium, trehalose biosynthesis, and different types of carbohydrate-active enzymes (CAZymes). These enzymes are crucial for nutraceutical and agro-industrial applications of . The multiple-step proteomic approach used could be a model for investigating other fungal species.
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http://dx.doi.org/10.3390/jof11020120 | DOI Listing |
J Dairy Res
March 2025
Department of Food Technology, Technological Federal University of Paraná, Londrina, Pioneiros Avenue 3131, Jardim Morumbi, 86036-370 Londrina, Paraná, Brazil.
This research paper presents the characterization of an enterocin-producing MF5 isolate and the determination of the in vitro antilisterial activity of enterocin produced by this isolate, named Ent-MF5. PCR-based screening for bacteriocin biosynthetic genes revealed that MF5 harbors multiple enterocin-encoding genes ( A, B, P and X), classified as class II bacteriocins and enterocin-P of (sharing up to 99% similarity at the genetic level). MF5 is sensitive to eight clinically important antibiotics and does not possess cytolysin activator -A, gelatinase -E and hyaluronidase -lA virulence genes.
View Article and Find Full Text PDFMycoKeys
March 2025
State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture, Collection and Application, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China South China Agricultural University Guangzhou China.
Three new species of are discovered from southern China. is characterized by its small basidiomata, greenish-green pileus, slender stipe, narrow, and close lamellae. is characterized by its pubescent pileus when young, yellowish white to pale yellow lamellae that are lighter towards the margin, narrowly cylindrical to lageniform pleurocystidia, and absent cheilocystidia.
View Article and Find Full Text PDFMycoKeys
March 2025
School of Public Health, Zunyi Medical University, Zunyi City, Guizhou Province 563000, China.
and are reported from China and identified through DNA sequence analyses (LSU, ITS, and 1-α) and morphological characteristics. Phylogenetic analysis showed that forms a distinct lineage within Mytilinidiaceae, closely related to the subclade, leading to the establishment of a new genus within this family. This saprotrophic species grows on the bark of , often surrounded by the thallus of species.
View Article and Find Full Text PDFMycoKeys
March 2025
Evolution and Conservation Biology, University of Liège, Sart Tilman B22, 4000 Liège, Belgium University of Liège Liege Belgium.
Two additional species of are described as new to science based on material from Bolivia and Peru and supported by phylogenetic analysis of the fungal ITS barcoding marker. The two new species represent lineages within clade I on the global phylogeny. Ossowska, B.
View Article and Find Full Text PDFFront Immunol
March 2025
Specialised Microbiology and Laboratories, United Kingdom Health Security Agency (UKHSA), Salisbury, Wiltshire, United Kingdom.
Introduction: Nipah virus (NiV) is one of a group of highly pathogenic viruses classified within the Henipavirus genus. Since 2012 at least 11 new henipa-like viruses have been identified, including from new locations and reservoir hosts; the pathogenicity of these new viruses has yet to be determined, but two of them have been associated with morbidity, including fatalities.
Methods: The efficacy and cross-reactivity of two vaccine candidates derived from the soluble glycoproteins of both NiV and Hendra virus (HeV) was evaluated in our recently established hamster model.
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