Purpose: This study aimed to investigate the epidemiology and dissemination of -positive in urban sewage in Yangzhou, China.
Methods: A total of 366 sewage samples were collected from the Yangzhou Wastewater Treatment Plant in Jiangsu Province. Colistin-resistant was identified through PCR targeting to genes. The isolates underwent antimicrobial susceptibility testing, and whole-genome sequencing was performed to analyze their genomic features. Additionally, conjugation experiments were conducted to assess the transferability of -positive plasmids.
Results: Three -positive isolates were identified, representing an isolation rate of 0.82%. These included one -positive (ST167) and two -positive complex strains with novel sequence types ST6801 and ST6825. The gene was located on an IncI2 plasmid (pYZ22WS208_3) and successfully transferred to recipient strains. In contrast, the gene was carried on IncF plasmids (pYZ22WS067_1 and pYZ22WS223_1) but was not transferable in this study. Phylogenetic analysis revealed that the -positive strain clustered within Clade II, alongside strains from various countries and sources. Phylogenomic analysis of -positive isolates showed their sporadic distribution across 13 countries, with associations to diverse hosts and environments, indicating potential for widespread transmission.
Conclusion: This study demonstrates the presence of and -positive in wastewater, emphasizing the importance of wastewater surveillance for tracking antibiotic resistance. The horizontal transfer of and potential spread of across various hosts underscore the need for ongoing monitoring and preventive measures.
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| http://dx.doi.org/10.2147/IDR.S502067 | DOI Listing |
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