The protein kinase A/protein kinase G/protein kinase C-family (AGC kinase family) of eukaryotes is involved in regulating numerous biological processes. The 3-phosphoinositide- dependent protein kinase 1 (PDK1), is a conserved serine/threonine kinase in eukaryotes. To understand the roles of homologous genes in cell death and immunity in tetraploid , the previuosly generated transgenic CRISPR/Cas9 lines, in which 5-7 alleles of the 4 homologous genes (/// homologs) simultaneously knocked out, were used in this study. Our results showed that the hypersensitive response (HR) triggered by transient overexpression of active Pto (Pto) or soybean MEKK1 was significantly delayed, whereas the resistance to pv. DC3000 ( DC3000) and tobacco mosaic virus (TMV) was significantly elevated in these partial knockout lines. The elevated resistance to DC3000 and TMV was correlated with the elevated activation of MPK6, MPK3, and MPK4. Taken together, our results indicated that 1s play a positive role in cell death but a positive role in disease resistance, likely through negative regulation of the MAPK signaling cascade.
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