Ribonucleoprotein (RNP) complexes can form multiple mesoscale assemblies, including stress granules (SGs). However, the function and regulation of the soluble RNP complexes are not fully understood. A recent study by Boraas et al. showed that G3BP1, a key node in SG formation, forms focal adhesion (FA)-localized RNP complexes and regulates cell migration.
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http://dx.doi.org/10.1016/j.tcb.2025.02.001 | DOI Listing |
iScience
March 2025
School of Medical Sciences, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
Existing research has proven difficult to understand the interplay between upstream signaling events during NLRP3 inflammasome activation. Additionally, events downstream of inflammasome complex formation such as cytokine release and pyroptosis can exhibit variation, further complicating matters. Cell Painting has emerged as a prominent tool for unbiased evaluation of the effect of perturbations on cell morphological phenotypes.
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February 2025
Department of Biotechnology, University of Verona, Strada Le Grazie 15, Verona, Italy.
CRISPR/Cas9 genome editing technology has revolutionized plant breeding by offering precise and rapid modifications. Traditional breeding methods are often slow and imprecise, whereas CRISPR/Cas9 allows for targeted genetic improvements. Previously, direct delivery of Cas9-single guide RNA (sgRNA) ribonucleoprotein (RNP) complexes to grapevine () protoplasts has been demonstrated, but successful regeneration of edited protoplasts into whole plants has not been achieved.
View Article and Find Full Text PDFSemin Arthritis Rheum
March 2025
The First Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Kitakyushu 807-8555, Japan. Electronic address:
In 1972, mixed connective tissue disease (MCTD) was proposed by Sharp et al. as a disease entity characterized by overlapping clinical features of systemic lupus erythematosus, systemic sclerosis (SSc), and polymyositis, as well as high titers of serum anti-U1-RNP antibody. However, the disease concept of MCTD is sometimes far from being sufficiently acknowledged.
View Article and Find Full Text PDFMicrobiol Spectr
February 2025
Department of Forest & Conservation Sciences, Faculty of Forestry, University of British Columbia, Vancouver, British Columbia, Canada.
CRISPR-Cas9 gene editing has become an important tool for the study of plant pathogens, allowing researchers to functionally characterize specific genes involved in phytopathogenicity, virulence, and fungicide resistance. Protocols for CRISPR-Cas9 gene editing have already been developed for Phytophthoras, an important group of oomycete plant pathogens; however, these efforts have exclusively focused on agricultural pathosystems, with research lacking for forest pathosystems. We sought to develop CRISPR-Cas9 gene editing in two forest pathogenic Phytophthoras, and , using a plasmid-ribonucleoprotein (RNP) co-transformation approach.
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February 2025
The Centre for Phenogenomics, Toronto, Canada.
Mice carrying patient-associated base changes are powerful tools to define the causality of single-nucleotide variants to disease states. Epitope tags enable immuno-based studies of genes for which no antibodies are available. These alleles enable detailed and precise developmental, mechanistic, and translational research.
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