A method for the determination of cholesterol using rhodamine derivatives as chromogenic substrates was proposed in this study. The oxidation of rhodamine B hydrazide (RBH) by hydrogen peroxide (HO) was catalyzed by a peroxide-mimicking enzyme to produce the fluorescent product. The reaction was further combined with the oxidase system to achieve indirect determination of cholesterol concentration, which can be catalyzed by cholesterol oxidase to produce HO as an intermediate product. Optical fiber optofluidic lasers (FOFLs), as a sensitive biochemical detection platform, have the ability to distinguish small differences in the amount of fluorescence products generated by the reaction and were used to achieve sensitive cholesterol detection. Under optimized experimental conditions, the dynamic range of three orders of magnitude was obtained in the determination of HO with a lower limit of detection (LOD) of 1.63 μM, and the assay time was as short as 3.5 min. On this basis, the designed cholesterol sensor obtained a linear range of 2.55-652.67 μM with a lower LOD of 2.55 μM and proved to have good selectivity. The possibility of cholesterol determination in human serum was initially tested. The sensor was also characterized by easy signal collection, wash-free procedure, controlled sample consumption, fast assay, low cost, and simple operation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.analchem.4c06462 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
An In-Plane Single-Photon Emitter Combining a Triangular Split-Ring Micro-Optical Resonator and a Colloidal Quantum Dot.
Nanomaterials (Basel)
February 2025
Graduate School of Engineering Science, Yokohama National University, Yokohama 240-8501, Japan.
We propose a simple and innovative configuration consisting of a quantum dot and micro-optical resonator that emits single photons with good directionality in a plane parallel to the substrate. In this device, a single quantum dot is placed as a light source between the slits of a triangular split-ring micro-optical resonator (SRR) supported in an optical polymer film with an air-bridge structure. Although most of the previous single photon emitters in solid-state devices emitted photons upward from the substrate, operation simulations confirmed that this configuration realizes lateral light emission in narrow regions above, below, left, and right in the optical polymer film, despite the absence of a light confinement structure such as an optical waveguide.
View Article and Find Full Text PDFFractal Dimension Analysis of Peripapillary Microvasculature in Primary Congenital Glaucoma.
J Glaucoma
March 2025
Department of Ophthalmology, Harvey and Bernice Jones Eye Institute, University of Arkansas for Medical Sciences, Little Rock, AR.
Precis: In this prospective multicenter study, eyes with primary congenital glaucoma exhibited lower retinal vascular parameters compared to healthy controls, as assessed by optical coherence tomography angiography fractal dimension analysis.
Purpose: To study the retino-choroidal peripapillary microvascular pattern in primary congenital glaucoma (PCG) using fractal dimension (FD) analysis and compare it to healthy controls.
Methods: This was a prospective multicenter comparative study.
Social Vulnerability Associated with Frequency of Visual Field and Optical Coherence Tomography Testing in Glaucoma Patients.
J Glaucoma
March 2025
Department of Ophthalmology, University of Washington, Seattle, WA, USA.
Precis: Higher Social Vulnerability Index scores, limited-English proficiency and retirement/disability status were associated with lower odds of completing one visual field and optical coherence tomography/year in glaucoma patients.
Purpose: To assess the correlation between sociodemographic factors and the Social Vulnerability Index to the likelihood of not receiving one visual field testing and one retinal nerve fiber layer optical coherence tomography test per year.
Methods: Patient records with glaucoma living in Washington state from a single academic institution (2014-2021) were reviewed.
Visible light polarization-sensitive optical coherence tomography with balanced detection.
J Biomed Opt
March 2025
University of Minnesota, Department of Biomedical Engineering, Minneapolis, Minnesota, United States.
Significance: We introduce a visible-light polarization-sensitive optical coherence tomography (PS-OCT) system that operates in the spectral domain with balanced detection (BD) capability. While the BD improves the signal-to-noise ratio (SNR), the use of shorter wavelengths improves spatial resolution and birefringence sensitivity.
Aim: We aim to implement a new optical design, characterize its performance, and investigate the imaging potential for biological tissues.
Optical coherence tomography needle probe for real-time visualization of temperature-induced phase changes within subcutaneous fatty tissue.
J Biomed Opt
March 2025
Universität zu Lübeck, Institute of Biomedical Optics, Lübeck, Germany.
: Selective cryolipolysis is a widely used aesthetic procedure that cools subcutaneous adipose tissue to temperatures as low as to induce fat cell destruction. However, real-time monitoring techniques are lacking, limiting the ability to optimize safety and efficacy. Traditional imaging methods either fail to provide adequate penetration depth or lack the resolution necessary for visualizing subcutaneous fatty tissue dynamics.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!