An intracellular protein ligation system using a truncated variant of AEP1 (AEP1 core) to ligate and polymerize Ig domains in is developed, enabling real-time polyprotein synthesis without enzyme activation and external ligation. This approach yields functional, mechanically stable polyproteins and expands AEP applications in synthetic biology and biomaterials.
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| http://dx.doi.org/10.1039/d4cc06617k | DOI Listing |
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