is an apicomplexan protozoal parasite that was first recognized during a mass mortality event in juvenile grey seals () in the northwest Atlantic Ocean. Since its identification, this parasite has been reported in various pinniped species and has been associated with fatal necrotizing hepatitis. Little is known of the host range of . Here we report 2 cases of California sea lions () in managed care that died following an 8-d history of inappetence, vomiting, diarrhea, and progressive lethargy with elevated hepatic enzyme activities. Postmortem examination identified hepatitis and icterus. schizonts and zoites were identified in regions of necrosis. Molecular and ultrastructural findings demonstrated the close relatedness of this to , which produces a similar lesion in bears.
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http://dx.doi.org/10.1177/10406387251321832 | DOI Listing |
Nat Commun
March 2025
Institute for Biochemistry and Molecular Biology, University of Hamburg, 20146, Hamburg, Germany.
During bacterial translation initiation, the 30S ribosomal subunit, initiation factors, and initiator tRNA define the reading frame of the mRNA. This process is inhibited by kasugamycin, edeine and GE81112, however, their mechanisms of action have not been fully elucidated. Here we present cryo-electron microscopy structures of 30S initiation intermediate complexes formed in the presence of kasugamycin, edeine and GE81112 at resolutions of 2.
View Article and Find Full Text PDFThe γ-tubulin ring complex (γ-TuRC) acts as a structural template for microtubule formation at centrosomes, associating with two main compartments: the pericentriolar material and the centriole lumen. In the pericentriolar material, the γ-TuRC is involved in microtubule organization, while the function of the centriole lumenal pool remains unclear. The conformational landscape of the γ-TuRC, which is crucial for its activity, and its centrosomal anchoring mechanisms, which determine γ-TuRC activity and turnover, are not understood.
View Article and Find Full Text PDFPoult Sci
March 2025
Department of Animal Science, National Chung Hsing University, Taichung 40227, Taiwan; The iEGG and Animal Biotechnology Center and Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung 40227, Taiwan; i-Center for Advanced Science and Technology (iCAST), National Chung Hsing University, Taichung 40227, Taiwan. Electronic address:
In mammals, tissues other than liver and intestine are known to possess functional MTTP (microsomal triglyceride transfer protein) and apoB (apolipoprotein B) capable of VLDL (very low-density lipoprotein) assembly. Birds are oviparous and possess unique capabilities in lipid biology to accommodate yolk formation through massive deposition of hepatically assembled yolk-targeted VLDLy into ovarian follicles. Following identifications of MTTP and ApoB expression within chicken ovarian stroma, granulosa, theca, and epithelial cells of various classes of follicles, we sought to define the functionality of ovarian MTTP and ApoB in VLDL assembly.
View Article and Find Full Text PDFRice (N Y)
March 2025
Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Agricultural College of Yangzhou University, Yangzhou, 225009, China.
Chloroplasts are vital for photosynthesis, and their development necessitates proper expression of chloroplast genes. However, the regulatory mechanisms underlying rice chloroplast gene expression have not been fully elucidated. In this study, we obtained an albino mutant of rice, white seedling and lethal 1 (wsl1), which displays significantly decreased chlorophyll contents and impaired chloroplast ultrastructure.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
March 2025
Department of Biology, Johns Hopkins University, Baltimore, MD 21218.
Microsporidia are divergent fungal pathogens that employ a unique harpoon-like apparatus called the polar tube (PT) to invade host cells. The long PT is fired out of the microsporidian spore over the course of just a few hundred milliseconds. Once fired, the PT is thought to pierce the plasma membrane of a target cell and act as a conduit for the transfer of the parasite into the host cell, which initiates infection.
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